AF4370

antibody from R&D Systems

Targeting: ATF1 TREB36

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF4370

Provider

R&D Systems

Product name

Human/Mouse ATF1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human and mouse ATF1 in Western blots.

Reactivity

Human, Mouse

Host

Goat

Conjugate

Unconjugated

Antigen sequence

P18846


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human/Mouse ATF1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and NIH-3T3 mouse embryonic fibroblast cell line. Gels were loaded with 30 μg of whole cell lysate (WCL), 20 μg of cytoplasmic (Cyto), and 10 μg of nuclear extracts (Nuc). PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse ATF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4370) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band for ATF1 was detected at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.