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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- MA5-24341 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BSSP4 Monoclonal Antibody (725445)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse Tryptase epsilon /BSSP-4 is observed.
- Antibody clone number
- 725445
- Concentration
- 0.2 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of human pancreas tissue. PVDF membrane was probed with 2 µg/mL of mouse Anti-human Tryptase e/BSSP-4 Monoclonal Antibody (Product # MA5-24341) followed by HRP-conjugated Anti-mouse IgG Secondary Antibody. A specific band was detected for Tryptase e/BSSP-4 at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Tryptase e/BSSP-4 was detected in immersion fixed paraffin-embedded sections of human pancreas using mouse Anti-human Tryptase e/BSSP-4 Monoclonal Antibody (Product # MA5-24341) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval. Tissue was stained using the Anti-mouse HRP-DAB (brown and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in exocrine cells.
