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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- 609-401-E07 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#609-401-E07, RRID:AB_11183024
- Product name
- Anti-Parkin pS378 (Rabbit) Antibody - 609-401-E07
- Antibody type
- Polyclonal
- Vial size
- 100 µl
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Supportive validation
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- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western Blot of Rabbit anti-Parkin pS378 antibody. Lane 1: HEK293 cells transfected with Parkin WT (Phospho). Lane 2: HEK293 cells transfected with Parkin S378 mutant (non-phospho). Load: 10 µg per lane. Primary antibody: Parkin pS378 antibody at 1:1,000 for overnight at 4°C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 52 kDa for Parkin pS378. Other band(s): none.
- Validation comment
- Western Blot
