AF2726

antibody from R&D Systems

Targeting: LMO2 RBTN2, RBTNL1, RHOM2, TTG2

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF2726

Provider

R&D Systems

Product name

Human LMO2 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human LMO2 in direct ELISAs and Western blots.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Antigen sequence

P25791


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

LMO2 is required for TAL1 DNA binding activity and initiation of definitive haematopoiesis at the haemangioblast stage.

Stanulovic VS, Cauchy P, Assi SA, Hoogenkamp M
Nucleic acids research 2017 Sep 29;45(17):9874-9888

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Reprogramming mouse fibroblasts into engraftable myeloerythroid and lymphoid progenitors.

Cheng H, Ang HY, A El Farran C, Li P, Fang HT, Liu TM, Kong SL, Chin ML, Ling WY, Lim EK, Li H, Huber T, Loh KM, Loh YH, Lim B
Nature communications 2016 Nov 21;7:13396

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Interleukin-7 receptor mutants initiate early T cell precursor leukemia in murine thymocyte progenitors with multipotent potential.

Treanor LM, Zhou S, Janke L, Churchman ML, Ma Z, Lu T, Chen SC, Mullighan CG, Sorrentino BP
The Journal of experimental medicine 2014 Apr 7;211(4):701-13

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Aberrant induction of LMO2 by the E2A-HLF chimeric transcription factor and its implication in leukemogenesis of B-precursor ALL with t(17;19).

Hirose K, Inukai T, Kikuchi J, Furukawa Y, Ikawa T, Kawamoto H, Oram SH, Göttgens B, Kiyokawa N, Miyagawa Y, Okita H, Akahane K, Zhang X, Kuroda I, Honna H, Kagami K, Goi K, Kurosawa H, Look AT, Matsui H, Inaba T, Sugita K
Blood 2010 Aug 12;116(6):962-70

Immunocytochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

LMO2 in K562 Human Cell Line. LMO2 was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using Human LMO2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2726) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of LMO2 in K562 Human Cell Line by Flow Cytometry. K562 chronic myelogenous leukemia human cell line was stained with Goat Anti-Human LMO2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2726, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Membrane-associated Proteins.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of LMO2 in Human PBMC by Flow Cytometry Human PBMC were stained with Goat Anti-Human LMO2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2726, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Membrane-associated Proteins.