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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- MAB10672 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB10672, RRID:AB_11189825
- Product name
- GSK3B monoclonal antibody, clone 3D10
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant GSK3B.
- Isotype
- IgG
- Antibody clone number
- 3D10
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis using GSK3B monoclonal antibody, clone 3D10 (Cat # MAB10672) against A-549 (1) , K-562 (2) , PC-12 (3) , NIH/3T3 (4) , and HEK293 (5) cell lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of NIH/3T3 (upper) and U251 (bottom) cells using GSK3B monoclonal antibody, clone 3D10 (Cat # MAB10672) (green) . Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human lung cancer (upper) and breast cancer tissues (bottom) using GSK3B monoclonal antibody, clone 3D10 (Cat # MAB10672) with DAB staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HeLa cells using GSK3B monoclonal antibody, clone 3D10 (Cat # MAB10672) (green) and negative control (purple) .
- Validation comment
- Flow Cytometry
