Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- BS6365 - Provider product page
- Provider
- Bioworld Technology, Inc
- Product name
- GSK3ÉÇ (phospho S9) polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic phosphopeptide derived from human GSK3ÉÇ around the phosphorylation site of Serine 9.
- Description
- Glycogen synthase kinase-3Ï«(GSK-3Ï«) and GSK-3ÉÇ are highly similar isoforms of serine/ threonine kinases that regulate metabolic enzymes and transcription factors, which are responsible for coordinating processes such as glycogen synthesis and cell adhesion. GSK-3ÉÇ activity is also required for nuclear activity of Rel dimers, which mediate an anti-apoptotic response to TNFÏ« in mice. GSK-3 catalytic kinase activity is controlled through differential phosphorylation of serine/threonine residues, which have an inhibitory effect, and tyrosine residues, which have an activating effect. Growth factor stimulation of mammalian cells expressing GSK-3Ï« and GSK-3ÉÇ induces phosphorylation of Ser 21 and Ser 9, respectively, through a phosphatidylinositol 3-kinase (PI 3-K)-protein kinase B (PKB)-dependent pathway, thereby enhancing proliferative signals. Additionally, GSK-3 physically associates with cAMP-dependent protein kinase A (PKA), which phosphorylates Ser 21 of GSK-3Ï« or Ser 9 of GSK-3ÉÇ and inactivates both forms. GSK-3Ï«/ÉÇ is positively regulated by phosphorylation on Tyr 279 and Tyr 216, respectively. Activated GSK-3Ï«/ÉÇ participates in energy metabolism, neuronal cell development, and body pattern formation. Tyrosine dephosphorylation of GSK-3 is involved in its extracellular signal-dependent inactivation.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Bioworld Technology, Inc (provider)
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- Experimental details
- WesternBlot (WB) analysis of GSK3¦Â (phospho-S9) polyclonal antibody