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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- F54007 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- GSK3 beta Antibody / GSK3B
- Antibody type
- Monoclonal
- Description
- Purified
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 2E6-D6-C12
- Vial size
- 0.1 ml
- Storage
- Store the GSK3B antibody at -20°C.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human A549, human HeLa, mouse NIH3T3 and rat brain lysates using GSK3B antibody at 1:1000. Predicted molecular weight ~46 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ICC/IF staining of HeLa cells (fixed with -20oC Methanol) with GSK3B antibody 1:200.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunoprecipitation of GSK3 beta from HeLa cell lysate using the GSK3B antibody. The precipitate was subsequently western blot tested with the same mAb at 1:1000.
