GTX83315

antibody from GeneTex

Targeting: GSK3B

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX83315

Provider

GeneTex

Proper citation

GeneTex Cat#GTX83315, RRID:AB_11171858

Product name

GSK3 beta antibody [3D10]

Antibody type

Monoclonal

Reactivity

Human, Mouse, Rat, Simian

Host

Mouse

References

Submitted references

GSK3β negatively regulates TRAX, a scaffold protein implicated in mental disorders, for NHEJ-mediated DNA repair in neurons.

Chien T, Weng YT, Chang SY, Lai HL, Chiu FL, Kuo HC, Chuang DM, Chern Y
Molecular psychiatry 2018 Dec;23(12):2375-2390

---

GSK3β expression and phosphorylation during neuronal maturation in the rat dorsal root ganglion.

Tang W, Han N, Liu Y, Li Z, Wei Y
International journal of clinical and experimental medicine 2015;8(4):5897-903

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Western blot analysis using GSK3B mAb against A549 (1), K562 (2), PC-12 (3), NIH/3T3 (4), and HEK293 (5) cell lysate.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunofluorescence analysis of NIH/3T3 (left) and U251 (right) cells using GSK3B mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human lung cancer (left) and breast cancer tissues (right) using GSK3B mAb with DAB staining.

Flow cytometry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Flow cytometric analysis of Hela cells using GSK3B mouse mAb (green) and negative control (purple)