MAB2164-100

antibody from R&D Systems

Targeting: CXCR2 CD182, CMKAR2, IL8RB

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB2164-100

Provider

R&D Systems

Product name

Mouse CXCR2/IL-8RB Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Stains mouse CXCR2 transfected cells but not CXCR1 transfectants.

Reactivity

Mouse

Host

Rat

Conjugate

Unconjugated

Antigen sequence

P35343


Isotype

IgG

Antibody clone number

242216

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Secreted nuclear protein DEK regulates hematopoiesis through CXCR2 signaling.

Capitano ML, Mor-Vaknin N, Saha AK, Cooper S, Legendre M, Guo H, Contreras-Galindo R, Kappes F, Sartor MA, Lee CT, Huang X, Markovitz DM, Broxmeyer HE
The Journal of clinical investigation 2019 May 20;129(6):2555-2570

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Estrogen stimulates female cancer progression by inducing myeloid-derived suppressive cells: investigations on pregnant and non-pregnant experimental models.

Kozasa K, Mabuchi S, Matsumoto Y, Kuroda H, Yokoi E, Komura N, Kawano M, Takahashi R, Sasano T, Shimura K, Kodama M, Hashimoto K, Sawada K, Nagasaka K, Kimura T
Oncotarget 2019 Mar 8;10(20):1887-1902

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Novel adherent CD11b⁺ Gr-1⁺ tumor-infiltrating cells initiate an immunosuppressive tumor microenvironment.

Tsubaki T, Kadonosono T, Sakurai S, Shiozawa T, Goto T, Sakai S, Kuchimaru T, Sakamoto T, Watanabe H, Kondoh G, Kizaka-Kondoh S
Oncotarget 2018 Feb 16;9(13):11209-11226

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The mTOR signal regulates myeloid-derived suppressor cells differentiation and immunosuppressive function in acute kidney injury.

Zhang C, Wang S, Li J, Zhang W, Zheng L, Yang C, Zhu T, Rong R
Cell death & disease 2017 Mar 23;8(3):e2695

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Microbiome-mediated neutrophil recruitment via CXCR2 and protection from amebic colitis.

Watanabe K, Gilchrist CA, Uddin MJ, Burgess SL, Abhyankar MM, Moonah SN, Noor Z, Donowitz JR, Schneider BN, Arju T, Ahmed E, Kabir M, Alam M, Haque R, Pramoonjago P, Mehrad B, Petri WA Jr
PLoS pathogens 2017 Aug;13(8):e1006513

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Sphingolipid pathway regulates innate immune responses at the fetomaternal interface during pregnancy.

Mizugishi K, Inoue T, Hatayama H, Bielawski J, Pierce JS, Sato Y, Takaori-Kondo A, Konishi I, Yamashita K
The Journal of biological chemistry 2015 Jan 23;290(4):2053-68

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The calcineurin-NFAT axis controls allograft immunity in myeloid-derived suppressor cells through reprogramming T cell differentiation.

Wang X, Bi Y, Xue L, Liao J, Chen X, Lu Y, Zhang Z, Wang J, Liu H, Yang H, Liu G
Molecular and cellular biology 2015 Feb;35(3):598-609

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CXCR2-mediated tumor-associated neutrophil recruitment is regulated by IFN-β.

Jablonska J, Wu CF, Andzinski L, Leschner S, Weiss S
International journal of cancer 2014 Mar 15;134(6):1346-58

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mTOR limits the recruitment of CD11b+Gr1+Ly6Chigh myeloid-derived suppressor cells in protecting against murine immunological hepatic injury.

Zhang Y, Bi Y, Yang H, Chen X, Liu H, Lu Y, Zhang Z, Liao J, Yang S, Chu Y, Yang R, Liu G
Journal of leukocyte biology 2014 Jun;95(6):961-70

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Fibrates ameliorate the course of bacterial sepsis by promoting neutrophil recruitment via CXCR2.

Tancevski I, Nairz M, Duwensee K, Auer K, Schroll A, Heim C, Feistritzer C, Hoefer J, Gerner RR, Moschen AR, Heller I, Pallweber P, Li X, Theurl M, Demetz E, Wolf AM, Wolf D, Eller P, Ritsch A, Weiss G
EMBO molecular medicine 2014 Jun;6(6):810-20

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Platelets guide the formation of early metastatic niches.

Labelle M, Begum S, Hynes RO
Proceedings of the National Academy of Sciences of the United States of America 2014 Jul 29;111(30):E3053-61

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IL-17A produced by neutrophils protects against pneumonic plague through orchestrating IFN-γ-activated macrophage programming.

Bi Y, Zhou J, Yang H, Wang X, Zhang X, Wang Q, Wu X, Han Y, Song Y, Tan Y, Du Z, Yang H, Zhou D, Cui Y, Zhou L, Yan Y, Zhang P, Guo Z, Wang X, Liu G, Yang R
Journal of immunology (Baltimore, Md. : 1950) 2014 Jan 15;192(2):704-13

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Phosphatase Wip1 negatively regulates neutrophil migration and inflammation.

Sun B, Hu X, Liu G, Ma B, Xu Y, Yang T, Shi J, Yang F, Li H, Zhang L, Zhao Y
Journal of immunology (Baltimore, Md. : 1950) 2014 Feb 1;192(3):1184-95

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Dendritic cells: In vitro culture in two- and three-dimensional collagen systems and expression of collagen receptors in tumors and atherosclerotic microenvironments.

Sprague L, Muccioli M, Pate M, Singh M, Xiong C, Ostermann A, Niese B, Li Y, Li Y, Courreges MC, Benencia F
Experimental cell research 2014 Apr 15;323(1):7-27

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Inefficient antimicrobial functions of innate phagocytes render infant mice more susceptible to bacterial infection.

Zhang Q, Coveney AP, Yu S, Liu JH, Li Y, Blankson S, Redmond HP, Wang JH, Wang J
European journal of immunology 2013 May;43(5):1322-32

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G protein-coupled receptor kinase 6 deficiency promotes angiogenesis, tumor progression, and metastasis.

Raghuwanshi SK, Smith N, Rivers EJ, Thomas AJ, Sutton N, Hu Y, Mukhopadhyay S, Chen XL, Leung T, Richardson RM
Journal of immunology (Baltimore, Md. : 1950) 2013 May 15;190(10):5329-36

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Respiratory syncytial virus induces airway insensitivity to beta-agonists in BALB/c mice.

Traylor ZP, Yu EN, Davis IC
American journal of physiology. Lung cellular and molecular physiology 2010 Mar;298(3):L437-45

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Neuroprotection and remyelination after autoimmune demyelination in mice that inducibly overexpress CXCL1.

Omari KM, Lutz SE, Santambrogio L, Lira SA, Raine CS
The American journal of pathology 2009 Jan;174(1):164-76

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Prostaglandin mediates IL-23/IL-17-induced neutrophil migration in inflammation by inhibiting IL-12 and IFNgamma production.

Lemos HP, Grespan R, Vieira SM, Cunha TM, Verri WA Jr, Fernandes KS, Souto FO, McInnes IB, Ferreira SH, Liew FY, Cunha FQ
Proceedings of the National Academy of Sciences of the United States of America 2009 Apr 7;106(14):5954-9

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An age-associated increase in pulmonary inflammation after burn injury is abrogated by CXCR2 inhibition.

Nomellini V, Faunce DE, Gomez CR, Kovacs EJ
Journal of leukocyte biology 2008 Jun;83(6):1493-501

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Chemokine-mediated rapid turnover of myeloid-derived suppressor cells in tumor-bearing mice.

Sawanobori Y, Ueha S, Kurachi M, Shimaoka T, Talmadge JE, Abe J, Shono Y, Kitabatake M, Kakimi K, Mukaida N, Matsushima K
Blood 2008 Jun 15;111(12):5457-66

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HIF-1alpha regulates epithelial inflammation by cell autonomous NFkappaB activation and paracrine stromal remodeling.

Scortegagna M, Cataisson C, Martin RJ, Hicklin DJ, Schreiber RD, Yuspa SH, Arbeit JM
Blood 2008 Apr 1;111(7):3343-54

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Genetic removal of the A2A adenosine receptor enhances pulmonary inflammation, mucin production, and angiogenesis in adenosine deaminase-deficient mice.

Mohsenin A, Mi T, Xia Y, Kellems RE, Chen JF, Blackburn MR
American journal of physiology. Lung cellular and molecular physiology 2007 Sep;293(3):L753-61

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An essential role for IL-17 in preventing pathogen-initiated bone destruction: recruitment of neutrophils to inflamed bone requires IL-17 receptor-dependent signals.

Yu JJ, Ruddy MJ, Wong GC, Sfintescu C, Baker PJ, Smith JB, Evans RT, Gaffen SL
Blood 2007 May 1;109(9):3794-802

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

CXCR2/IL-8 RB in Mouse Spleen. CXCR2/IL-8 RB was detected in perfusion fixed frozen sections of mouse spleen using Rat Anti-Mouse CXCR2/IL-8 RB Monoclonal Antibody (Catalog # MAB2164) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell sufaces in splenocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of CXCR2/IL-8 RB in HEK293 Human Cell Line Transfected with Mouse CXCR2/IL-8 RB and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) mouse CXCR2/IL-8 RB or (B) mouse CXCR1/IL-8 RA and eGFP was stained with Rat Anti-Mouse CXCR2/IL-8 RB Monoclonal Antibody (Catalog # MAB2164) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). Quadrant markers were set based on control antibody staining (Catalog # MAB006).

Blocking/Neutralizing

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Chemotaxis Induced by CXCL2/MIP-2 and Neutralization by Mouse CXCR2/IL-8 RB Antibody. Recombinant Mouse CXCL2/MIP-2 (Catalog # 452-M2) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL2/MIP-2 (2 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CXCR2/IL-8 RB Monoclonal Antibody (Catalog # MAB2164). The ND₅₀ is typically 15-50 µg/mL.