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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [44]
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- Validations
- Immunohistochemistry [1]
- Flow cytometry [1]
- Blocking/Neutralizing [1]
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- Product number
- MAB331-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CXCR2/IL-8RB Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human CXCR2/IL-8 RB transfected NS0 cells but not the parental cell line. It does not cross-react with CXCR1 (IL-8 RA).
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
AAB25880- Isotype
- IgG
- Antibody clone number
- 48311
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references A Subset of Paracrine Factors as Efficient Biomarkers for Predicting Vascular Regenerative Efficacy of Mesenchymal Stromal/Stem Cells.
CXCR2-Expressing Tumor Cells Drive Vascular Mimicry in Antiangiogenic Therapy-Resistant Glioblastoma.
IL-8/CXCR1/2 signalling promotes tumor cell proliferation, invasion and vascular mimicry in glioblastoma.
Mycobacteria Manipulate G-Protein-Coupled Receptors to Increase Mucosal Rac1 Expression in the Lungs.
Clinicopathologic significance of the CXCL1-CXCR2 axis in the tumor microenvironment of gastric carcinoma.
Proteomic profiling of peripheral blood neutrophils identifies two inflammatory phenotypes in stable COPD patients.
Adoptive Transfer of Phosphoantigen-Specific γδ T Cell Subset Attenuates Mycobacterium tuberculosis Infection in Nonhuman Primates.
Evidence for chemokine synergy during neutrophil migration in ARDS.
Cancer cell-secreted IGF2 instigates fibroblasts and bone marrow-derived vascular progenitor cells to promote cancer progression.
Role of Autophagy in HIV-1 Matrix Protein p17-Driven Lymphangiogenesis.
TNF-α augments CXCR2 and CXCR3 to promote progression of renal cell carcinoma.
Tumor-derived interleukin-1 promotes lymphangiogenesis and lymph node metastasis through M2-type macrophages.
Hypoxia promotes the proliferation of cervical carcinoma cells through stimulating the secretion of IL-8.
Simian immunodeficiency virus and human immunodeficiency virus type 1 matrix proteins specify different capabilities to modulate B cell growth.
CXCL1/CXCL8 (GROα/IL-8) in human diabetic ketoacidosis plasma facilitates leukocyte recruitment to cerebrovascular endothelium in vitro.
The role of CXC-chemokine receptor CXCR2 and suppressor of cytokine signaling-3 (SOCS-3) in renal cell carcinoma.
Expansion of activated regulatory T cells by myeloid-specific chemokines via an alternative pathway in CSF of bacterial meningitis patients.
CD56(bright)perforin(low) noncytotoxic human NK cells are abundant in both healthy and neoplastic solid tissues and recirculate to secondary lymphoid organs via afferent lymph.
Interleukin-33 overexpression is associated with gamma-glutamyl transferase in biliary atresia.
Glioblastoma stem cells are regulated by interleukin-8 signaling in a tumoral perivascular niche.
Overexpression of the β subunit of human chorionic gonadotropin promotes the transformation of human ovarian epithelial cells and ovarian tumorigenesis.
Overexpression of CXCL5 is associated with poor survival in patients with pancreatic cancer.
Reduced expression of chemoattractant receptors by polymorphonuclear leukocytes in Hyper IgE Syndrome patients.
Primary human acute myelogenous leukemia cells release matrix metalloproteases and their inhibitors: release profile and pharmacological modulation.
IL8 and CXCL13 are potent chemokines for the recruitment of human neural precursor cells across brain endothelial cells.
CXCR2 promotes ovarian cancer growth through dysregulated cell cycle, diminished apoptosis, and enhanced angiogenesis.
Spatiotemporal expression of chemokines and chemokine receptors in experimental anti-myeloperoxidase antibody-mediated glomerulonephritis.
CXCR1 and CXCR2 enhances human melanoma tumourigenesis, growth and invasion.
Expression of CXCL8, CXCR1, and CXCR2 in neurons and glial cells of the human and rabbit retina.
Potential role of high-mobility group box 1 in cystic fibrosis airway disease.
TSU68 prevents liver metastasis of colon cancer xenografts by modulating the premetastatic niche.
Synergy between coproduced CC and CXC chemokines in monocyte chemotaxis through receptor-mediated events.
A novel proteolytic cascade generates an extracellular matrix-derived chemoattractant in chronic neutrophilic inflammation.
Cytokine and chemokine profiles in multiple myeloma; significance of stromal interaction and correlation of IL-8 production with disease progression.
Role of interleukin-8 and growth-regulated oncogene-alpha in the chemotactic migration of all-trans retinoic acid-treated promyelocytic leukemic cells toward alveolar epithelial cells.
Increased levels of neutrophil-activating peptide-2 in acute coronary syndromes: possible role of platelet-mediated vascular inflammation.
IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis.
Chemokine receptor expression in rat adjuvant-induced arthritis.
The activation of IL-8 receptors in cultured guinea pig Müller glial cells is modified by signals from retinal pigment epithelium.
Specific CXC but not CC chemokines cause elevated monocyte migration in COPD: a role for CXCR2.
Possible link between unique chemokine and homing receptor expression at diagnosis and relapse location in a patient with childhood T-ALL.
Expression of functional CCR and CXCR chemokine receptors in podocytes.
Imbalance in the expression of CXC chemokines correlates with bronchoalveolar lavage fluid angiogenic activity and procollagen levels in acute respiratory distress syndrome.
Unique subpopulations of CD56+ NK and NK-T peripheral blood lymphocytes identified by chemokine receptor expression repertoire.
Kim HK, Lee SG, Lee SW, Oh BJ, Kim JH, Kim JA, Lee G, Jang JD, Joe YA
Stem cells (Dayton, Ohio) 2019 Jan;37(1):77-88
Stem cells (Dayton, Ohio) 2019 Jan;37(1):77-88
CXCR2-Expressing Tumor Cells Drive Vascular Mimicry in Antiangiogenic Therapy-Resistant Glioblastoma.
Angara K, Borin TF, Rashid MH, Lebedyeva I, Ara R, Lin PC, Iskander A, Bollag RJ, Achyut BR, Arbab AS
Neoplasia (New York, N.Y.) 2018 Oct;20(10):1070-1082
Neoplasia (New York, N.Y.) 2018 Oct;20(10):1070-1082
IL-8/CXCR1/2 signalling promotes tumor cell proliferation, invasion and vascular mimicry in glioblastoma.
Sharma I, Singh A, Siraj F, Saxena S
Journal of biomedical science 2018 Aug 8;25(1):62
Journal of biomedical science 2018 Aug 8;25(1):62
Mycobacteria Manipulate G-Protein-Coupled Receptors to Increase Mucosal Rac1 Expression in the Lungs.
Alaridah N, Lutay N, Tenland E, Rönnholm A, Hallgren O, Puthia M, Westergren-Thorsson G, Godaly G
Journal of innate immunity 2017;9(3):318-329
Journal of innate immunity 2017;9(3):318-329
Clinicopathologic significance of the CXCL1-CXCR2 axis in the tumor microenvironment of gastric carcinoma.
Kasashima H, Yashiro M, Nakamae H, Masuda G, Kinoshita H, Morisaki T, Fukuoka T, Hasegawa T, Nakane T, Hino M, Hirakawa K, Ohira M
PloS one 2017;12(6):e0178635
PloS one 2017;12(6):e0178635
Proteomic profiling of peripheral blood neutrophils identifies two inflammatory phenotypes in stable COPD patients.
Loi ALT, Hoonhorst S, van Aalst C, Langereis J, Kamp V, Sluis-Eising S, Ten Hacken N, Lammers JW, Koenderman L
Respiratory research 2017 May 22;18(1):100
Respiratory research 2017 May 22;18(1):100
Adoptive Transfer of Phosphoantigen-Specific γδ T Cell Subset Attenuates Mycobacterium tuberculosis Infection in Nonhuman Primates.
Qaqish A, Huang D, Chen CY, Zhang Z, Wang R, Li S, Yang E, Lu Y, Larsen MH, Jacobs WR Jr, Qian L, Frencher J, Shen L, Chen ZW
Journal of immunology (Baltimore, Md. : 1950) 2017 Jun 15;198(12):4753-4763
Journal of immunology (Baltimore, Md. : 1950) 2017 Jun 15;198(12):4753-4763
Evidence for chemokine synergy during neutrophil migration in ARDS.
Williams AE, José RJ, Mercer PF, Brealey D, Parekh D, Thickett DR, O'Kane C, McAuley DF, Chambers RC
Thorax 2017 Jan;72(1):66-73
Thorax 2017 Jan;72(1):66-73
Cancer cell-secreted IGF2 instigates fibroblasts and bone marrow-derived vascular progenitor cells to promote cancer progression.
Xu WW, Li B, Guan XY, Chung SK, Wang Y, Yip YL, Law SY, Chan KT, Lee NP, Chan KW, Xu LY, Li EM, Tsao SW, He QY, Cheung AL
Nature communications 2017 Feb 10;8:14399
Nature communications 2017 Feb 10;8:14399
Role of Autophagy in HIV-1 Matrix Protein p17-Driven Lymphangiogenesis.
Mazzuca P, Marsico S, Schulze K, Mitola S, Pils MC, Giagulli C, Guzman CA, Caruso A, Caccuri F
Journal of virology 2017 Aug 15;91(16)
Journal of virology 2017 Aug 15;91(16)
TNF-α augments CXCR2 and CXCR3 to promote progression of renal cell carcinoma.
Sun KH, Sun GH, Wu YC, Ko BJ, Hsu HT, Wu ST
Journal of cellular and molecular medicine 2016 Nov;20(11):2020-2028
Journal of cellular and molecular medicine 2016 Nov;20(11):2020-2028
Tumor-derived interleukin-1 promotes lymphangiogenesis and lymph node metastasis through M2-type macrophages.
Watari K, Shibata T, Kawahara A, Sata K, Nabeshima H, Shinoda A, Abe H, Azuma K, Murakami Y, Izumi H, Takahashi T, Kage M, Kuwano M, Ono M
PloS one 2014;9(6):e99568
PloS one 2014;9(6):e99568
Hypoxia promotes the proliferation of cervical carcinoma cells through stimulating the secretion of IL-8.
Liu LB, Xie F, Chang KK, Li MQ, Meng YH, Wang XH, Li H, Li DJ, Yu JJ
International journal of clinical and experimental pathology 2014;7(2):575-83
International journal of clinical and experimental pathology 2014;7(2):575-83
Simian immunodeficiency virus and human immunodeficiency virus type 1 matrix proteins specify different capabilities to modulate B cell growth.
Caccuri F, Giagulli C, Reichelt J, Martorelli D, Marsico S, Bugatti A, Barone I, Rusnati M, Guzman CA, Dolcetti R, Caruso A
Journal of virology 2014 May;88(10):5706-17
Journal of virology 2014 May;88(10):5706-17
CXCL1/CXCL8 (GROα/IL-8) in human diabetic ketoacidosis plasma facilitates leukocyte recruitment to cerebrovascular endothelium in vitro.
Omatsu T, Cepinskas G, Clarson C, Patterson EK, Alharfi IM, Summers K, Couraud PO, Romero IA, Weksler B, Fraser DD, Canadian Critical Care Translational Biology Group.
American journal of physiology. Endocrinology and metabolism 2014 May 1;306(9):E1077-84
American journal of physiology. Endocrinology and metabolism 2014 May 1;306(9):E1077-84
The role of CXC-chemokine receptor CXCR2 and suppressor of cytokine signaling-3 (SOCS-3) in renal cell carcinoma.
Stofas A, Levidou G, Piperi C, Adamopoulos C, Dalagiorgou G, Bamias A, Karadimou A, Lainakis GA, Papadoukakis S, Stravodimos K, Dimopoulos MA, Patsouris E, Gakiopoulou H, Korkolopoulou P
BMC cancer 2014 Mar 4;14:149
BMC cancer 2014 Mar 4;14:149
Expansion of activated regulatory T cells by myeloid-specific chemokines via an alternative pathway in CSF of bacterial meningitis patients.
Shi G, Han J, Liu G, Hao Y, Ma Y, Li T, Wu X, Zhang H, Liu Y, Wang B, Kong Y, Zhou J, Zeng H
European journal of immunology 2014 Feb;44(2):420-30
European journal of immunology 2014 Feb;44(2):420-30
CD56(bright)perforin(low) noncytotoxic human NK cells are abundant in both healthy and neoplastic solid tissues and recirculate to secondary lymphoid organs via afferent lymph.
Carrega P, Bonaccorsi I, Di Carlo E, Morandi B, Paul P, Rizzello V, Cipollone G, Navarra G, Mingari MC, Moretta L, Ferlazzo G
Journal of immunology (Baltimore, Md. : 1950) 2014 Apr 15;192(8):3805-15
Journal of immunology (Baltimore, Md. : 1950) 2014 Apr 15;192(8):3805-15
Interleukin-33 overexpression is associated with gamma-glutamyl transferase in biliary atresia.
Dong R, Dong K, Wang X, Chen G, Shen C, Zheng S
Cytokine 2013 Feb;61(2):433-7
Cytokine 2013 Feb;61(2):433-7
Glioblastoma stem cells are regulated by interleukin-8 signaling in a tumoral perivascular niche.
Infanger DW, Cho Y, Lopez BS, Mohanan S, Liu SC, Gursel D, Boockvar JA, Fischbach C
Cancer research 2013 Dec 1;73(23):7079-89
Cancer research 2013 Dec 1;73(23):7079-89
Overexpression of the β subunit of human chorionic gonadotropin promotes the transformation of human ovarian epithelial cells and ovarian tumorigenesis.
Guo X, Liu G, Schauer IG, Yang G, Mercado-Uribe I, Yang F, Zhang S, He Y, Liu J
The American journal of pathology 2011 Sep;179(3):1385-93
The American journal of pathology 2011 Sep;179(3):1385-93
Overexpression of CXCL5 is associated with poor survival in patients with pancreatic cancer.
Li A, King J, Moro A, Sugi MD, Dawson DW, Kaplan J, Li G, Lu X, Strieter RM, Burdick M, Go VL, Reber HA, Eibl G, Hines OJ
The American journal of pathology 2011 Mar;178(3):1340-9
The American journal of pathology 2011 Mar;178(3):1340-9
Reduced expression of chemoattractant receptors by polymorphonuclear leukocytes in Hyper IgE Syndrome patients.
Mintz R, Garty BZ, Meshel T, Marcus N, Katanov C, Cohen-Hillel E, Ben-Baruch A
Immunology letters 2010 May 4;130(1-2):97-106
Immunology letters 2010 May 4;130(1-2):97-106
Primary human acute myelogenous leukemia cells release matrix metalloproteases and their inhibitors: release profile and pharmacological modulation.
Reikvam H, Hatfield KJ, Oyan AM, Kalland KH, Kittang AO, Bruserud O
European journal of haematology 2010 Mar;84(3):239-51
European journal of haematology 2010 Mar;84(3):239-51
IL8 and CXCL13 are potent chemokines for the recruitment of human neural precursor cells across brain endothelial cells.
Weiss N, Deboux C, Chaverot N, Miller F, Baron-Van Evercooren A, Couraud PO, Cazaubon S
Journal of neuroimmunology 2010 Jun;223(1-2):131-4
Journal of neuroimmunology 2010 Jun;223(1-2):131-4
CXCR2 promotes ovarian cancer growth through dysregulated cell cycle, diminished apoptosis, and enhanced angiogenesis.
Yang G, Rosen DG, Liu G, Yang F, Guo X, Xiao X, Xue F, Mercado-Uribe I, Huang J, Lin SH, Mills GB, Liu J
Clinical cancer research : an official journal of the American Association for Cancer Research 2010 Aug 1;16(15):3875-86
Clinical cancer research : an official journal of the American Association for Cancer Research 2010 Aug 1;16(15):3875-86
Spatiotemporal expression of chemokines and chemokine receptors in experimental anti-myeloperoxidase antibody-mediated glomerulonephritis.
van der Veen BS, Petersen AH, Belperio JA, Satchell SC, Mathieson PW, Molema G, Heeringa P
Clinical and experimental immunology 2009 Oct;158(1):143-53
Clinical and experimental immunology 2009 Oct;158(1):143-53
CXCR1 and CXCR2 enhances human melanoma tumourigenesis, growth and invasion.
Singh S, Nannuru KC, Sadanandam A, Varney ML, Singh RK
British journal of cancer 2009 May 19;100(10):1638-46
British journal of cancer 2009 May 19;100(10):1638-46
Expression of CXCL8, CXCR1, and CXCR2 in neurons and glial cells of the human and rabbit retina.
Goczalik I, Ulbricht E, Hollborn M, Raap M, Uhlmann S, Weick M, Pannicke T, Wiedemann P, Bringmann A, Reichenbach A, Francke M
Investigative ophthalmology & visual science 2008 Oct;49(10):4578-89
Investigative ophthalmology & visual science 2008 Oct;49(10):4578-89
Potential role of high-mobility group box 1 in cystic fibrosis airway disease.
Rowe SM, Jackson PL, Liu G, Hardison M, Livraghi A, Solomon GM, McQuaid DB, Noerager BD, Gaggar A, Clancy JP, O'Neal W, Sorscher EJ, Abraham E, Blalock JE
American journal of respiratory and critical care medicine 2008 Oct 15;178(8):822-31
American journal of respiratory and critical care medicine 2008 Oct 15;178(8):822-31
TSU68 prevents liver metastasis of colon cancer xenografts by modulating the premetastatic niche.
Yamamoto M, Kikuchi H, Ohta M, Kawabata T, Hiramatsu Y, Kondo K, Baba M, Kamiya K, Tanaka T, Kitagawa M, Konno H
Cancer research 2008 Dec 1;68(23):9754-62
Cancer research 2008 Dec 1;68(23):9754-62
Synergy between coproduced CC and CXC chemokines in monocyte chemotaxis through receptor-mediated events.
Gouwy M, Struyf S, Noppen S, Schutyser E, Springael JY, Parmentier M, Proost P, Van Damme J
Molecular pharmacology 2008 Aug;74(2):485-95
Molecular pharmacology 2008 Aug;74(2):485-95
A novel proteolytic cascade generates an extracellular matrix-derived chemoattractant in chronic neutrophilic inflammation.
Gaggar A, Jackson PL, Noerager BD, O'Reilly PJ, McQuaid DB, Rowe SM, Clancy JP, Blalock JE
Journal of immunology (Baltimore, Md. : 1950) 2008 Apr 15;180(8):5662-9
Journal of immunology (Baltimore, Md. : 1950) 2008 Apr 15;180(8):5662-9
Cytokine and chemokine profiles in multiple myeloma; significance of stromal interaction and correlation of IL-8 production with disease progression.
Kline M, Donovan K, Wellik L, Lust C, Jin W, Moon-Tasson L, Xiong Y, Witzig TE, Kumar S, Rajkumar SV, Lust JA
Leukemia research 2007 May;31(5):591-8
Leukemia research 2007 May;31(5):591-8
Role of interleukin-8 and growth-regulated oncogene-alpha in the chemotactic migration of all-trans retinoic acid-treated promyelocytic leukemic cells toward alveolar epithelial cells.
Tsai WH, Hsu HC, Lin CC, Ho CK, Kou YR
Critical care medicine 2007 Mar;35(3):879-85
Critical care medicine 2007 Mar;35(3):879-85
Increased levels of neutrophil-activating peptide-2 in acute coronary syndromes: possible role of platelet-mediated vascular inflammation.
Smith C, Damås JK, Otterdal K, Øie E, Sandberg WJ, Yndestad A, Waehre T, Scholz H, Endresen K, Olofsson PS, Halvorsen B, Gullestad L, Frøland SS, Hansson GK, Aukrust P
Journal of the American College of Cardiology 2006 Oct 17;48(8):1591-9
Journal of the American College of Cardiology 2006 Oct 17;48(8):1591-9
IL-17 enhances the net angiogenic activity and in vivo growth of human non-small cell lung cancer in SCID mice through promoting CXCR-2-dependent angiogenesis.
Numasaki M, Watanabe M, Suzuki T, Takahashi H, Nakamura A, McAllister F, Hishinuma T, Goto J, Lotze MT, Kolls JK, Sasaki H
Journal of immunology (Baltimore, Md. : 1950) 2005 Nov 1;175(9):6177-89
Journal of immunology (Baltimore, Md. : 1950) 2005 Nov 1;175(9):6177-89
Chemokine receptor expression in rat adjuvant-induced arthritis.
Haas CS, Martinez RJ, Attia N, Haines GK 3rd, Campbell PL, Koch AE
Arthritis and rheumatism 2005 Dec;52(12):3718-30
Arthritis and rheumatism 2005 Dec;52(12):3718-30
The activation of IL-8 receptors in cultured guinea pig Müller glial cells is modified by signals from retinal pigment epithelium.
Malgorzata Goczalik I, Raap M, Weick M, Milenkovic I, Heidmann J, Enzmann V, Wiedemann P, Reichenbach A, Francke M
Journal of neuroimmunology 2005 Apr;161(1-2):49-60
Journal of neuroimmunology 2005 Apr;161(1-2):49-60
Specific CXC but not CC chemokines cause elevated monocyte migration in COPD: a role for CXCR2.
Traves SL, Smith SJ, Barnes PJ, Donnelly LE
Journal of leukocyte biology 2004 Aug;76(2):441-50
Journal of leukocyte biology 2004 Aug;76(2):441-50
Possible link between unique chemokine and homing receptor expression at diagnosis and relapse location in a patient with childhood T-ALL.
Annels NE, Willemze AJ, van der Velden VH, Faaij CM, van Wering E, Sie-Go DM, Egeler RM, van Tol MJ, Révész T
Blood 2004 Apr 1;103(7):2806-8
Blood 2004 Apr 1;103(7):2806-8
Expression of functional CCR and CXCR chemokine receptors in podocytes.
Huber TB, Reinhardt HC, Exner M, Burger JA, Kerjaschki D, Saleem MA, Pavenstädt H
Journal of immunology (Baltimore, Md. : 1950) 2002 Jun 15;168(12):6244-52
Journal of immunology (Baltimore, Md. : 1950) 2002 Jun 15;168(12):6244-52
Imbalance in the expression of CXC chemokines correlates with bronchoalveolar lavage fluid angiogenic activity and procollagen levels in acute respiratory distress syndrome.
Keane MP, Donnelly SC, Belperio JA, Goodman RB, Dy M, Burdick MD, Fishbein MC, Strieter RM
Journal of immunology (Baltimore, Md. : 1950) 2002 Dec 1;169(11):6515-21
Journal of immunology (Baltimore, Md. : 1950) 2002 Dec 1;169(11):6515-21
Unique subpopulations of CD56+ NK and NK-T peripheral blood lymphocytes identified by chemokine receptor expression repertoire.
Campbell JJ, Qin S, Unutmaz D, Soler D, Murphy KE, Hodge MR, Wu L, Butcher EC
Journal of immunology (Baltimore, Md. : 1950) 2001 Jun 1;166(11):6477-82
Journal of immunology (Baltimore, Md. : 1950) 2001 Jun 1;166(11):6477-82
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CXCR2/IL-8 RB in Human Lymph Node. CXCR2/IL-8 RB was detected in immersion fixed paraffin-embedded sections of human lymph node using 15 µg/mL Mouse Anti-Human CXCR2/IL-8 RB Monoclonal Antibody (Catalog # MAB331) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of CXCR2/IL-8 RB in Human Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Mouse Anti-Human CXCR2/IL-8 RB Monoclonal Antibody (Catalog # MAB331, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Chemotaxis Induced by CXCL1/GRO alpha and Neutralization by Human CXCR2/IL-8 RB Antibody. Recombinant Human CXCL1/GRO alpha (Catalog # 275-GR) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CXCL1/GRO alpha (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CXCR2/IL-8 RB Monoclonal Antibody (Catalog # MAB331). The ND50 is typically 1-5 µg/mL.
