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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- MAB5748 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CD35 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human CD35 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant rat CSMD1, recombinant human (rh) CSMD2, rhCSMD3, rhHABP1/C1QBP, or rhComplement Factor H is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
CAA68755- Isotype
- IgG
- Antibody clone number
- 594708
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of CD35 in Human PBMCs by Flow Cytometry. Human peripheral blood lymphocytes (PBMC) were stained with Human CD35 Monoclonal Antibody (Catalog # MAB5748) followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0101B) and Human CD19 PE-conjugated Monoclonal Antibody (Catalog # FAB4867P). Quadrant markers were set based on control antibody staining (Catalog # MAB002 and IC002P, respectively).
