PAB21240

antibody from Abnova Corporation

Targeting: ALYREF ALY, ALY/REF, BEF, REF, THOC4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB21240

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB21240, RRID:AB_10965715

Product name

ALYREF polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant ALYREF.

Antigen sequence

EFGTLKKAAVHYDRSGRSLGTADVHFERKADALKA
MKQYNGVPLDGRPMNIQLVTSQIDAQRRPAQSVNR
GGMTRNRGAGGFGGGGGTRRGTRGGARGRGRGAGR
NSKQQLSAEELDAQLDAYNARMD


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with ALYREF polyclonal antibody (Cat # PAB21240) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with ALYREF polyclonal antibody (Cat # PAB21240) at 1-4 ug/mL dilution shows positivity in nuclei but not nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human testis with ALYREF polyclonal antibody (Cat # PAB21240) shows strong nuclear positivity in cells in seminiferus tubules at 1:200-1:500 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)