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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-22314 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ALY Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2, Mouse brain. Predicted reactivity: Mouse (96%), Rat (98%), Chicken (82%), Rhesus Monkey (98%), Bovine (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.63 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ALY using 30 µg of MOLT4 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with an ALY polyclonal antibody (Product # PA5-22314) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ALY was performed by separating 30 µg of various whole cell extracts by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a ALY Polyclonal Antibody (Product # PA5-22314) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ALY Polyclonal Antibody (Product # PA5-22314). Sample (50 µg of whole cell lysate). Lane A: mouse brain. 12% SDS PAGE. ALY Polyclonal Antibody (Product # PA5-22314) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ALY in paraformaldehyde-fixed HeLa cells using an ALY polyclonal antibody (Product # PA5-22314) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of ALY was performed in paraffin-embedded human cervical carcinoma tissue using ALY Polyclonal Antibody (Product # PA5-22314) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of ALY was performed in paraffin-embedded human esophageal cancer tissue using ALY Polyclonal Antibody (Product # PA5-22314) at a dilution of 1:500.
