656603

antibody from BioLegend

Targeting: INPP5D hp51CN, SHIP, SHIP1

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

656603

Provider

BioLegend

Proper citation

BioLegend Cat#656603, RRID:AB_2562867

Product name

PE anti-SHIP-1

Antibody type

Monoclonal

Antigen

Human SHIP fusion protein in CFA/IFA

Description

SH2-containing inositol phosphatase 1 (SHIP1) is a hematopoietic phosphatase that hydrolyzes key signaling lipid PI(3,4,5)P(3) to PI(3,4)P(2). SHIP-1 is located in the cytoplasm and is recruited to the cell surface upon receptor ligation. It plays critical roles in hematopoietic cell proliferation, activation, differentiation, and survival through regulating PI3K and Akt pathways. Aberrant regulation of PI3K signaling can induce autoimmune diseases and cancer formation.

Reactivity

Human, Mouse

Host

Mouse

Conjugate

Yellow dye

Antibody clone number

P1C1-A5

Vial size

25 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Flow cytometry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

C57BL/6 mouse bone marrow cells were stimulated with (solid line) or without (filled histogram) LPS for 48 hours, then fixed and permeabilized with BioLegend Fixation Buffer (Cat. No. 420801) and Permeabilization Wash Buffer (Cat. No. 421002). Cells were then stained with SHIP-11 (clone P1C1-A5) PE or mouse IgG1, κ PE isotype control (dash line for unstimulated, dot line for stimulated). Data shown were gated on myeloid cell population.

Conjugate

Yellow dye