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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- sc-8425 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-8425, RRID:AB_628250
- Product name
- Anti-INPP5D
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
Submitted references Tumor necrosis factor induces GSK3 kinase-mediated cross-tolerance to endotoxin in macrophages.
Essential roles for Dok2 and RasGAP in CD200 receptor-mediated regulation of human myeloid cells.
SHIP prevents lipopolysaccharide from triggering an antiviral response in mice.
Tolerance induced via TLR2 and TLR4 in human dendritic cells: role of IRAK-1.
The inositol phosphatase SHIP controls Salmonella enterica serovar Typhimurium infection in vivo.
Compound heterozygosity for Pten and SHIP augments T-dependent humoral immune responses and cytokine production by CD(4+) T cells.
Park SH, Park-Min KH, Chen J, Hu X, Ivashkiv LB
Nature immunology 2011 May 22;12(7):607-15
Nature immunology 2011 May 22;12(7):607-15
Essential roles for Dok2 and RasGAP in CD200 receptor-mediated regulation of human myeloid cells.
Mihrshahi R, Barclay AN, Brown MH
Journal of immunology (Baltimore, Md. : 1950) 2009 Oct 15;183(8):4879-86
Journal of immunology (Baltimore, Md. : 1950) 2009 Oct 15;183(8):4879-86
SHIP prevents lipopolysaccharide from triggering an antiviral response in mice.
Sly LM, Hamilton MJ, Kuroda E, Ho VW, Antignano FL, Omeis SL, van Netten-Thomas CJ, Wong D, Brugger HK, Williams O, Feldman ME, Houseman BT, Fiedler D, Shokat KM, Krystal G
Blood 2009 Mar 26;113(13):2945-54
Blood 2009 Mar 26;113(13):2945-54
Tolerance induced via TLR2 and TLR4 in human dendritic cells: role of IRAK-1.
Albrecht V, Hofer TP, Foxwell B, Frankenberger M, Ziegler-Heitbrock L
BMC immunology 2008 Nov 24;9:69
BMC immunology 2008 Nov 24;9:69
The inositol phosphatase SHIP controls Salmonella enterica serovar Typhimurium infection in vivo.
Bishop JL, Sly LM, Krystal G, Finlay BB
Infection and immunity 2008 Jul;76(7):2913-22
Infection and immunity 2008 Jul;76(7):2913-22
Compound heterozygosity for Pten and SHIP augments T-dependent humoral immune responses and cytokine production by CD(4+) T cells.
Moody JL, Jirik FR
Immunology 2004 Jul;112(3):404-12
Immunology 2004 Jul;112(3):404-12
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Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Single band corresponding to the predicted size in kDa (+/-20%).
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:7000
- Lane 1
- Marker [kDa]: 250, 130, 95, 72, 55, 36, 28, 17, 11
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 128
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in lymphoid cells.
- Validation comment
- Staining pattern consistent with experimental and/or bioinformatic data.
