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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- sc-9967 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-9967, RRID:AB_2096794
- Product name
- Anti-E2F2
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
Submitted references The retinoblastoma tumor suppressor controls androgen signaling and human prostate cancer progression.
Enterocyte proliferation and intestinal hyperplasia induced by simian virus 40 T antigen require a functional J domain.
Intestinal hyperplasia induced by simian virus 40 large tumor antigen requires E2F2.
Sharma A, Yeow WS, Ertel A, Coleman I, Clegg N, Thangavel C, Morrissey C, Zhang X, Comstock CE, Witkiewicz AK, Gomella L, Knudsen ES, Nelson PS, Knudsen KE
The Journal of clinical investigation 2010 Dec;120(12):4478-92
The Journal of clinical investigation 2010 Dec;120(12):4478-92
Enterocyte proliferation and intestinal hyperplasia induced by simian virus 40 T antigen require a functional J domain.
Rathi AV, Sáenz Robles MT, Pipas JM
Journal of virology 2007 Sep;81(17):9481-9
Journal of virology 2007 Sep;81(17):9481-9
Intestinal hyperplasia induced by simian virus 40 large tumor antigen requires E2F2.
Sáenz-Robles MT, Markovics JA, Chong JL, Opavsky R, Whitehead RH, Leone G, Pipas JM
Journal of virology 2007 Dec;81(23):13191-9
Journal of virology 2007 Dec;81(23):13191-9
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Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- No bands detected.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:7000
- Lane 1
- Marker [kDa]: 250, 130, 95, 72, 55, 36, 28, 17, 11
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 48
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human placenta shows moderate cytoplasmic and nuclear positivity in trophoblastic cells.
- Validation comment
- Staining pattern partly consistent with experimental and/or bioinformatic data.
