H00001870-D01P

antibody from Abnova Corporation

Targeting: E2F2 E2F-2

Western blot (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00001870-D01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00001870-D01P, RRID:AB_1673869

Product name

E2F2 purified MaxPab rabbit polyclonal antibody (D01P)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human E2F2 protein.

Antigen sequence

MLQGPRALASAAGQTPKVVPAMSPTELWPSGLSSP
QLCPATATYYTPLYPQTAPPAAAPGTCLDATPHGP
EGQVVRCLPAGRLPAKRKLDLEGIGRPVVPEFPTP
KGKCIRVDGLPSPKTPKSPGEKTRYDTSLGLLTKK
FIYLLSESEDGVLDLNWAAEVLDVQKRRIYDITNV
LEGIQLIRKKAKNNIQWVGRGMFEDPTRPGKQQQL
GQELKELMNTEQALDHLIQSCSLSFKHLTEDKANK
RLAYVTYQDIRAVGNFKEQTVIAVKAPPQTRLEVP
DRTEDNLQIYLKSTQGPIEVYLCPEEVQEPDSPSE
EPLPSTSTLCPSPDSAQPSSSTDPSIMEPTASSVP
APAPTPQQAPPPPSLVPLEATDSLLELPHPLLQQT
EDQFLSPTLACSSPLISFSPSLDQDDYLWGLEAGE
GISDLFDSYDLGDLLIN


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of E2F2 expression in transfected 293T cell line (H00001870-T04) by E2F2 MaxPab polyclonal antibody.Lane 1: E2F2 transfected lysate(47.50 KDa).Lane 2: Non-transfected lysate.

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between E2F2 and E2F1. HeLa cells were stained with anti-E2F2 rabbit purified polyclonal 1:1200 and anti-E2F1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)