sc-33638

antibody from Santa Cruz Biotechnology

Targeting: CDC73 C1orf28, FIHP, HRPT1, HRPT2, parafibromin

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

sc-33638

Provider

Santa Cruz Biotechnology

Proper citation

Santa Cruz Biotechnology Cat#sc-33638, RRID:AB_628102

Product name

Anti-CDC73

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

References

Submitted references

Downregulated parafibromin expression is a promising marker for pathogenesis, invasion, metastasis and prognosis of gastric carcinomas.

Zheng HC, Takahashi H, Li XH, Hara T, Masuda S, Guan YF, Takano Y
Virchows Archiv : an international journal of pathology 2008 Feb;452(2):147-55

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Immunohistochemical assessment of parafibromin in mouse and human tissues.

Porzionato A, Macchi V, Barzon L, Masi G, Iacobone M, Parenti A, Palù G, De Caro R
Journal of anatomy 2006 Dec;209(6):817-27

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

Single band corresponding to the predicted size in kDa (+/-20%).

Primary Ab dilution

1:500

Secondary Ab dilution

1:7000

Lane 1

Marker [kDa]: 250, 130, 95, 72, 55, 36, 28, 17, 11

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

Human Plasma

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 29

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human colon shows nuclear positivity in glandular cells.

Validation comment

Staining pattern consistent with experimental and/or bioinformatic data.