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Learn12310-1-AP
antibody from Invitrogen Antibodies
Targeting: CDC73
C1orf28, FIHP, HRPT1, HRPT2, parafibromin
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- 12310-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HRPT2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: WRTRTTILQ STGKNFSKNI FAILQSVKAR EEGRAPEQRP APNAAPVDPT LRTKQPIPAA YNRYDQERFK GKEETEGFKI DTMGTYHGMT LKSVTEGASA RKTQTPAAQP VPRPVSQARP PPNQKKGSRT PIIIIPAATT SLITMLNAKD LLQDLKFVPS DEKKKQGCQR ENETLIQRRK DQMQPGGTAI SVTVPYRVVD QPLKLMPQDW DRVVAVFVQG PAWQFKGWPW LLPDGSPVDI FAKIKAFHLK YDEVRLDPNV QKWDVTVLEL SYHKRHLDRP VFLRFWETLD RYMVKHKSHL RF (232-531 aa encoded by BC014351)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.13 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human lung tissue were subjected to SDS PAGE followed by western blot with 12310-1-AP (HRPT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WB result of HRPT2 antibody (12310-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HRPT2 transfected HEK-293 cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse testis tissue were subjected to SDS PAGE followed by western blot with 12310-1-AP (HRPT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human heart tissue were subjected to SDS PAGE followed by western blot with 12310-1-AP (HRPT2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HRPT2, CDC73 antibody (Product # 12310-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using CDC73 antibody 12310-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IGG (green). Blue pseudocolor = DAPI (fluorescent DNA dye).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human breast cancer using 12310-1-AP (HRPT2 antibody) at dilution of 1:50 (under 10x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-HRPT2; CDC73 (IP:12310-1-AP, 4ug; Detection:12310-1-AP 1:500) with mouse testis tissue lysate 4000ug.
