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LearnNB200-184
antibody from Novus Biologicals
Targeting: CDC73
C1orf28, FIHP, HRPT1, HRPT2, parafibromin
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [10]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
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- Product number
- NB200-184 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB200-184, RRID:AB_2078391
- Product name
- Rabbit Polyclonal CDC73/HRPT2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. NB200-184 is specific for human Parafibromin protein.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Histone H3R17me2a mark recruits human RNA polymerase-associated factor 1 complex to activate transcription.
The 68 kDa subunit of mammalian cleavage factor I interacts with the U7 small nuclear ribonucleoprotein and participates in 3'-end processing of animal histone mRNAs.
The tumor suppressor, parafibromin, mediates histone H3 K9 methylation for cyclin D1 repression.
The tumor suppressor Cdc73 functionally associates with CPSF and CstF 3' mRNA processing factors.
The parafibromin tumor suppressor protein inhibits cell proliferation by repression of the c-myc proto-oncogene.
Parafibromin, a component of the human PAF complex, regulates growth factors and is required for embryonic development and survival in adult mice.
Trabid, a new positive regulator of Wnt-induced transcription with preference for binding and cleaving K63-linked ubiquitin chains.
Parafibromin immunoreactivity: its use as an additional diagnostic marker for parathyroid tumor classification.
Parafibromin/Hyrax activates Wnt/Wg target gene transcription by direct association with beta-catenin/Armadillo.
The HRPT2 tumor suppressor gene product parafibromin associates with human PAF1 and RNA polymerase II.
Wu J, Xu W
Proceedings of the National Academy of Sciences of the United States of America 2012 Apr 10;109(15):5675-80
Proceedings of the National Academy of Sciences of the United States of America 2012 Apr 10;109(15):5675-80
The 68 kDa subunit of mammalian cleavage factor I interacts with the U7 small nuclear ribonucleoprotein and participates in 3'-end processing of animal histone mRNAs.
Ruepp MD, Vivarelli S, Pillai RS, Kleinschmidt N, Azzouz TN, Barabino SM, Schümperli D
Nucleic acids research 2010 Nov;38(21):7637-50
Nucleic acids research 2010 Nov;38(21):7637-50
The tumor suppressor, parafibromin, mediates histone H3 K9 methylation for cyclin D1 repression.
Yang YJ, Han JW, Youn HD, Cho EJ
Nucleic acids research 2010 Jan;38(2):382-90
Nucleic acids research 2010 Jan;38(2):382-90
The tumor suppressor Cdc73 functionally associates with CPSF and CstF 3' mRNA processing factors.
Rozenblatt-Rosen O, Nagaike T, Francis JM, Kaneko S, Glatt KA, Hughes CM, LaFramboise T, Manley JL, Meyerson M
Proceedings of the National Academy of Sciences of the United States of America 2009 Jan 20;106(3):755-60
Proceedings of the National Academy of Sciences of the United States of America 2009 Jan 20;106(3):755-60
The parafibromin tumor suppressor protein inhibits cell proliferation by repression of the c-myc proto-oncogene.
Lin L, Zhang JH, Panicker LM, Simonds WF
Proceedings of the National Academy of Sciences of the United States of America 2008 Nov 11;105(45):17420-5
Proceedings of the National Academy of Sciences of the United States of America 2008 Nov 11;105(45):17420-5
Parafibromin, a component of the human PAF complex, regulates growth factors and is required for embryonic development and survival in adult mice.
Wang P, Bowl MR, Bender S, Peng J, Farber L, Chen J, Ali A, Zhang Z, Alberts AS, Thakker RV, Shilatifard A, Williams BO, Teh BT
Molecular and cellular biology 2008 May;28(9):2930-40
Molecular and cellular biology 2008 May;28(9):2930-40
Trabid, a new positive regulator of Wnt-induced transcription with preference for binding and cleaving K63-linked ubiquitin chains.
Tran H, Hamada F, Schwarz-Romond T, Bienz M
Genes & development 2008 Feb 15;22(4):528-42
Genes & development 2008 Feb 15;22(4):528-42
Parafibromin immunoreactivity: its use as an additional diagnostic marker for parathyroid tumor classification.
Juhlin CC, Villablanca A, Sandelin K, Haglund F, Nordenström J, Forsberg L, Bränström R, Obara T, Arnold A, Larsson C, Höög A
Endocrine-related cancer 2007 Jun;14(2):501-12
Endocrine-related cancer 2007 Jun;14(2):501-12
Parafibromin/Hyrax activates Wnt/Wg target gene transcription by direct association with beta-catenin/Armadillo.
Mosimann C, Hausmann G, Basler K
Cell 2006 Apr 21;125(2):327-41
Cell 2006 Apr 21;125(2):327-41
The HRPT2 tumor suppressor gene product parafibromin associates with human PAF1 and RNA polymerase II.
Yart A, Gstaiger M, Wirbelauer C, Pecnik M, Anastasiou D, Hess D, Krek W
Molecular and cellular biology 2005 Jun;25(12):5052-60
Molecular and cellular biology 2005 Jun;25(12):5052-60
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CDC73/HRPT2 Antibody [NB200-184] - Detection of Human Parafibromin on HeLa whole cell lysate using NB200-184. In B, Parafibromin was immunoprecipitated by using NB200-184 at 3 mcg/10mg with or without blocking peptide.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CDC73/HRPT2 Antibody [NB200-184] - Detection of Human and Mouse Para fibromin by Western Blot. Samples: Whole cell lysate (50 ug) from mouse TCMK-1 and human 293T cells. Antibodies: Affinity purified rabbit anti-Parafibromin antibody NB200-184 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: CDC73/HRPT2 Antibody [NB200-184] - Detection of human CDC73/HRPT2 (Parafibromin) by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-CDC73/HRPT2 antibody NB200-184 used for IP at 6 ug/mg lysate. For blotting immunoprecipitated CDC73/HRPT2, NB200-184 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
