PAB23326

antibody from Abnova Corporation

Targeting: HARBI1 C11orf77, FLJ32675

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB23326

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB23326, RRID:AB_11122578

Product name

HARBI1 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant HARBI1.

Antigen sequence

VIEKTFRTLCSRFRCLDGSKGALQYSPEKSSHIIL
ACCVLHNISLEHGMDVWSSPMTGPMEQPPEEEYEH
MESLDLEADRIRQELM


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) with HARBI1 polyclonal antibody (Cat # PAB23326) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251 MG with HARBI1 polyclonal antibody (Cat # PAB23326) at 1-4 ug/mL dilution shows positivity in cytoplasm.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human pancreas with HARBI1 polyclonal antibody (Cat # PAB23326) shows strong cytoplasmic positivity in exocrine glandular cells at 1:500-1:1000 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)