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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- NB120-11570 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB120-11570, RRID:AB_789291
- Product name
- Mouse Monoclonal Aggrecan Antibody
- Antibody type
- Monoclonal
- Description
- Unpurified. The antibody has been reported to be specific for the glycosaminoglycan (GAG) portion of native chondroitin sulfate proteoglycan (CSPG). The antibody reacts specifically with chondroitin sulfate types A and C but not with type B (dermatan sulfate), and may be used for localization of chondroitin sulfate in tissues and cultured fibroblasts.
- Reactivity
- Human, Mouse, Rat, Bovine, Chicken/Avian, Porcine, Rabbit
- Host
- Mouse
- Isotype
- IgM
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Responses of apoptosis and matrix metabolism of annulus fibrosus cells to different magnitudes of mechanical tension in vitro.
Static compression down-regulates N-cadherin expression and facilitates loss of cell phenotype of nucleus pulposus cells in a disc perfusion culture.
The response of nucleus pulposus cell senescence to static and dynamic compressions in a disc organ culture.
An interpenetrating network-strengthened and toughened hydrogel that supports cell-based nucleus pulposus regeneration.
Matrix homeostasis within the immature annulus fibrosus depends on the frequency of dynamic compression: a study based on the self-developed mechanically active bioreactor.
Long-term load duration induces N-cadherin down-regulation and loss of cell phenotype of nucleus pulposus cells in a disc bioreactor culture.
Biological Responses of the Immature Annulus Fibrosus to Dynamic Compression in a Disc Perfusion Culture.
Surgical removal and controlled trypsinization of the outer annulus fibrosus improves the bioactivity of the nucleus pulposus in a disc bioreactor culture.
Jiang Y, Fu L, Song Y
Bioscience reports 2019 Feb 28;39(2)
Bioscience reports 2019 Feb 28;39(2)
Static compression down-regulates N-cadherin expression and facilitates loss of cell phenotype of nucleus pulposus cells in a disc perfusion culture.
Zhou H, Shi J, Zhang C, Li P
Bioscience reports 2018 Feb 28;38(1)
Bioscience reports 2018 Feb 28;38(1)
The response of nucleus pulposus cell senescence to static and dynamic compressions in a disc organ culture.
Shi J, Pang L, Jiao S
Bioscience reports 2018 Apr 27;38(2)
Bioscience reports 2018 Apr 27;38(2)
An interpenetrating network-strengthened and toughened hydrogel that supports cell-based nucleus pulposus regeneration.
Gan Y, Li P, Wang L, Mo X, Song L, Xu Y, Zhao C, Ouyang B, Tu B, Luo L, Zhu L, Dong S, Li F, Zhou Q
Biomaterials 2017 Aug;136:12-28
Biomaterials 2017 Aug;136:12-28
Matrix homeostasis within the immature annulus fibrosus depends on the frequency of dynamic compression: a study based on the self-developed mechanically active bioreactor.
Li P, Gan Y, Xu Y, Song L, Wang H, Zhang C, Wang L, Zhao C, Luo L, Zhou Q
Biomechanics and modeling in mechanobiology 2017 Apr;16(2):385-394
Biomechanics and modeling in mechanobiology 2017 Apr;16(2):385-394
Long-term load duration induces N-cadherin down-regulation and loss of cell phenotype of nucleus pulposus cells in a disc bioreactor culture.
Li P, Zhang R, Wang L, Gan Y, Xu Y, Song L, Luo L, Zhao C, Zhang C, Ouyang B, Tu B, Zhou Q
Bioscience reports 2017 Apr 30;37(2)
Bioscience reports 2017 Apr 30;37(2)
Biological Responses of the Immature Annulus Fibrosus to Dynamic Compression in a Disc Perfusion Culture.
Li P, Gan Y, Wang H, Xu Y, Song L, Zhang C, Li S, Zhou Q
Cells, tissues, organs 2016;202(5-6):296-306
Cells, tissues, organs 2016;202(5-6):296-306
Surgical removal and controlled trypsinization of the outer annulus fibrosus improves the bioactivity of the nucleus pulposus in a disc bioreactor culture.
Li P, Shi R, Chen D, Gan Y, Xu Y, Song L, Li S, Zhou Q
BMC musculoskeletal disorders 2016 Mar 22;17:133
BMC musculoskeletal disorders 2016 Mar 22;17:133
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Frozen: Aggrecan Antibody (CS-56) [NB120-11570] - staining of a frozen section of normal rabbit aorta. Data supplied by Z. Gallis, McGill University, Montreal.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Aggrecan Antibody (CS-56) [NB120-11570] - Long-term load duration decreased expression of NP matrix macromolecules in porcine disc NP cells. Immunohistochemistry staining of aggrecan and collagen II. Magnification: 200x, scale =100 um. The results showed that long-term load duration (8 h per day) significantly decreased gene expression of matrix molecules (aggrecan and collagen II) and their protein deposition within the NP tissue compared with the short-term load duration (1 h per day) and the control (non-compression). Data are expressed as the means +/- S.D., n=3. #: indicates a significant difference (P
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Electron Microscopy: Aggrecan Antibody (CS-56) [NB120-11570] - Normal rabbit aorta, Lowicryl K4M thin section, stained with Monoclonal Anti-Chondroitin Sulfate (NB120-11570) and Goat Anti-Mouse IgM (u-chain specific) 10 nm gold. Counterstain was uranyl acetate and Reynold's lead citrate. Magnification 44,600x. (el=Elastin, col=Collagen). Data supplied by Z. Gallis, McGill University, Montreal.
