Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AF1220 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Aggrecan G1-IGD-G2 Domains Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Aggrecan in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
NP_037359
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Newly Defined ATP-Binding Cassette Subfamily B Member 5 Positive Dermal Mesenchymal Stem Cells Promote Healing of Chronic Iron-Overload Wounds via Secretion of Interleukin-1 Receptor Antagonist.
Effects of sesamin on chondroitin sulfate proteoglycan synthesis induced by interleukin-1beta in human chondrocytes.
Identification of Multipotent Stem Cells in Human Brain Tissue Following Stroke.
MSC from fetal and adult lungs possess lung-specific properties compared to bone marrow-derived MSC.
Human embryonic stem cell-derived mesenchymal stroma cells (hES-MSCs) engraft in vivo and support hematopoiesis without suppressing immune function: implications for off-the shelf ES-MSC therapies.
Age-related changes in rat bone-marrow mesenchymal stem cell plasticity.
Effect of functionalised fluorescence-labelled nanoparticles on mesenchymal stem cell differentiation.
Vander Beken S, de Vries JC, Meier-Schiesser B, Meyer P, Jiang D, Sindrilaru A, Ferreira FF, Hainzl A, Schatz S, Muschhammer J, Scheurmann NJ, Kampilafkos P, Seitz AM, Dürselen L, Ignatius A, Kluth MA, Ganss C, Wlaschek M, Singh K, Maity P, Frank NY, Frank MH, Scharffetter-Kochanek K
Stem cells (Dayton, Ohio) 2019 Aug;37(8):1057-1074
Stem cells (Dayton, Ohio) 2019 Aug;37(8):1057-1074
Effects of sesamin on chondroitin sulfate proteoglycan synthesis induced by interleukin-1beta in human chondrocytes.
Srisuthtayanont W, Pruksakorn D, Kongtawelert P, Pothacharoen P
BMC complementary and alternative medicine 2017 May 31;17(1):286
BMC complementary and alternative medicine 2017 May 31;17(1):286
Identification of Multipotent Stem Cells in Human Brain Tissue Following Stroke.
Tatebayashi K, Tanaka Y, Nakano-Doi A, Sakuma R, Kamachi S, Shirakawa M, Uchida K, Kageyama H, Takagi T, Yoshimura S, Matsuyama T, Nakagomi T
Stem cells and development 2017 Jun 1;26(11):787-797
Stem cells and development 2017 Jun 1;26(11):787-797
MSC from fetal and adult lungs possess lung-specific properties compared to bone marrow-derived MSC.
Rolandsson Enes S, Andersson Sjöland A, Skog I, Hansson L, Larsson H, Le Blanc K, Eriksson L, Bjermer L, Scheding S, Westergren-Thorsson G
Scientific reports 2016 Jul 6;6:29160
Scientific reports 2016 Jul 6;6:29160
Human embryonic stem cell-derived mesenchymal stroma cells (hES-MSCs) engraft in vivo and support hematopoiesis without suppressing immune function: implications for off-the shelf ES-MSC therapies.
Li O, Tormin A, Sundberg B, Hyllner J, Le Blanc K, Scheding S
PloS one 2013;8(1):e55319
PloS one 2013;8(1):e55319
Age-related changes in rat bone-marrow mesenchymal stem cell plasticity.
Asumda FZ, Chase PB
BMC cell biology 2011 Oct 12;12:44
BMC cell biology 2011 Oct 12;12:44
Effect of functionalised fluorescence-labelled nanoparticles on mesenchymal stem cell differentiation.
Tautzenberger A, Lorenz S, Kreja L, Zeller A, Musyanovych A, Schrezenmeier H, Landfester K, Mailänder V, Ignatius A
Biomaterials 2010 Mar;31(8):2064-71
Biomaterials 2010 Mar;31(8):2064-71
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Aggrecan in Rat Mesenchymal Stem Cells. Aggrecan was detected in immersion fixed rat mesenchymal stem cells differentiated to chondrocytes using Goat Anti-Human Aggrecan G1-IGD-G2 Domains Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1220) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conju-gated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Aggrecan in Human Cartilage. Aggrecan was detected in immersion fixed paraffin-embedded sections of human cartilage using 15 µg/mL Goat Anti-Human Aggrecan G1-IGD-G2 Domains Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1220) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.