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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
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- Product number
- MAB2197 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal ADAMTS1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human ADAMTS1 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human ADAMTS-L1.2 is observed.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Breast cancer cells induce stromal fibroblasts to secrete ADAMTS1 for cancer invasion through an epigenetic change.
ADAMTS9 is a cell-autonomously acting, anti-angiogenic metalloprotease expressed by microvascular endothelial cells.
Tyan SW, Hsu CH, Peng KL, Chen CC, Kuo WH, Lee EY, Shew JY, Chang KJ, Juan LJ, Lee WH
PloS one 2012;7(4):e35128
PloS one 2012;7(4):e35128
ADAMTS9 is a cell-autonomously acting, anti-angiogenic metalloprotease expressed by microvascular endothelial cells.
Koo BH, Coe DM, Dixon LJ, Somerville RP, Nelson CM, Wang LW, Young ME, Lindner DJ, Apte SS
The American journal of pathology 2010 Mar;176(3):1494-504
The American journal of pathology 2010 Mar;176(3):1494-504
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of Human ADAMTS1 by Western Blot. Western blot shows lysates of human ovarian cancer tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ADAMTS1 Monoclonal Antibody (Catalog # MAB2197) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ADAMTS1 at approximately 60-70 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 2.
