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LearnH00004154-M02
antibody from Abnova Corporation
Targeting: MBNL1
EXP, EXP35, EXP40, EXP42, KIAA0428, MBNL
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
Reference
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- Product number
- H00004154-M02 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00004154-M02, RRID:AB_509358
- Product name
- MBNL1 monoclonal antibody (M02), clone 3E7
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant MBNL1.
- Antigen sequence
MAVSVTPIRDTKWLTLEVCREFQRGTCSRPDTECK
FAHPSKSCQVENGRVIACFDSLKGRCSRENCKYLH
PPPHLKTQLEINGRNNLIQQKNMAMLAQQMQLANA
MMPGAPLQPVPMFSVAPSLATNASAAAFNPYLGPV
SPSLVPAEILPTAPMLVTGNPGVPVPAAAAAAAQK
LIRTDRLEVCREYQRGNCNRGENDCRFAHPADSTM
IDTNDNTVTVCMDYIKGRCSREKCKYFHPPAHLQA
KIKAAQYQVNQAAAAQAAATAAAMGIPQAVLPPLP
KRPALEKTNGATAVFNTGIFQYQQALANMQLQQHT
AFLPPGSILCMTPATSVVPMVHGATPATVSAATTS
ATSVPFAATATANQIPIISAEHLTSHKYVTQM- Isotype
- IgG
- Antibody clone number
- 3E7
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Development of an AP-FRET based analysis for characterizing RNA-protein interactions in myotonic dystrophy (DM1).
Rehman S, Gladman JT, Periasamy A, Sun Y, Mahadevan MS
PloS one 2014;9(4):e95957
PloS one 2014;9(4):e95957
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- MBNL1 monoclonal antibody (M02), clone 3E7 Western Blot analysis of MBNL1 expression in Hela S3 NE ( Cat # L013V3 ).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged MBNL1 is approximately 3ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to MBNL1 on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to MBNL1 on formalin-fixed paraffin-embedded human esophagus. [antibody concentration 0.75 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
