12780-1-AP

antibody from Invitrogen Antibodies

Targeting: CCP110 CP110, KIAA0419

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

12780-1-AP

Provider

Invitrogen Antibodies

Product name

CP110 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MEEYEKFCE KSLARIQEAS LSTESFLPAQ SESISLIRFH GVAILSPLLN IEKRKEMQQE KQKALDVEAR KQVNRKKALL TRVQEILDNV QVRKAPNASD FDQWEMETVY SNSEVRNLNV PATFPNSFPS HTEHSTAAKL DKIAGILPLD NEDQCKTDGI DLARDSEGFN SPKQCDSSNI SHVENEAFPK TSSATPQETL ISDGPFSVNE QQDLPLLAEV IPDPYVMSLQ NLMKKSKEYI EREQSRRSLR GSMNRIVNES HLDKEHDAVE VADCVKEKGQ LTGKHCVSVI PDKPSLNKSN VLLQGASTQA SSMSMPVLAS FSKVDIPIRT GHPTVLES (1-337 aa encoded by BC036654)

Reactivity

Human, Mouse, Rat, Canine

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.27 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 12780-1-AP (CP110 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

WB result of CP110 antibody (12780-1-AP, 1:500) with si-control and si-CP110 transfected HeLa cells.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

K-562 cells were subjected to SDS PAGE followed by western blot with 12780-1-AP (CP110 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IF result (Centrioles) of anti-CP110 (12780-1-AP) by Dr. Corbit Kevin.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human testis tissue slide using 12780-1-AP ( CP110 Antibody) at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human testis tissue slide using 12780-1-AP ( CP110 Antibody) at dilution of 1:200 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using 12780-1-AP (CP110 Antibody) at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using 12780-1-AP (CP110 Antibody) at dilution of 1:200 (under 40x lens).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 HeLa cells were stained with .2ug CP110 antibody (12780-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of CP110 (12780-1-AP) by Dr. Corbit, Kevin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-CP110 (IP:12780-1-AP, 3ug; Detection:12780-1-AP 1:1000) with HeLa cells lysate 3000ug.