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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [4]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- 10923-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- E2F4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MAEAGPQAP PPPGTPSRHE KSLGLLTTKF VSLLQEAKDG VLDLKLAADT LAVRQKRRIY DITNVLEGIG LIEKKSKNSI QWKGVGPGCN TREIADKLIE LKAEIEELQQ REQELDQHKV WVQQSIRNVT EDVQNSCLAY VTHEDICRCF AGDTLLAIRA PSGTSLEVPI PEGLNGQKKY QIHLKSVSGP IEVLLVNKEA WSSPPVAVPV PPPEDLLQSP SAVSTPPPLP KPALAQSQEA SRPNSPQLTP TAVPGSAEVQ GMAGPAAEIT VSGGPGTDSK DSGELSSLPL GPTTLDTRPL QSSALLDSSS SSSSSSSSSS NSNSSSSSGP NPSTSFEPIK ADPTGVLELP KELSEIFDPT RECMSSELLE ELMSSEVFAP LLRLSPPPGD HDYIYNLDES EGVCDLFDVP VLNL (1-413 aa encoded by BC033180)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.3 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10923-1-AP (E2F4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- A431 cells were subjected to SDS PAGE followed by western blot with 10923-1-AP (E2F4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HL-60 cells were subjected to SDS PAGE followed by western blot with 10923-1-AP (E2F4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Raji cells were subjected to SDS PAGE followed by western blot with 10923-1-AP (E2F4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lymphoma using 10923-1-AP (E2F4 antibody) at dilution of 1:100 (under 10x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-E2F4 (IP:10923-1-AP, 3ug; Detection:10923-1-AP 1:1000) with mouse brain tissue lysate 3600ug.
