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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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- Product number
- ARG54148 - Provider product page
- Provider
- Arigo
- Product name
- anti-RPA32 / RPA2 antibody
- Antibody type
- Monoclonal
- Antigen
- Purified recombinant human RPA32/RPA2 protein fragments expressed in E.coli.
- Description
- Affinity purified
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 3E7-B5-F2
- Vial size
- 100 µl
- Concentration
- 0.75 mg/ml
- Storage
- Aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: RPA32/RPA2 in MCF7, HeLa and HUVEC cell lysates stained with RPA32 / RPA2 antibody [3E7-B5-F2] (ARG54148) (diluted in 1: 2000). Predicted band size: 32KDa. Observed band size: 32KDa. Exposure time: 20s.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: HeLa cells fixed with -20¢J Methanol and stained with RPA32 / RPA2 antibody [3E7-B5-F2] (ARG54148) (diluted in 1:200).
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunoprecipitation: HeLa cell lysates immunoprecipitated and stained by RPA32 / RPA2 antibody [3E7-B5-F2] (ARG54148).
