Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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Validation data
Reference
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- Product number
- NBP2-22214 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Glut4 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references mTORC2 sustains thermogenesis via Akt-induced glucose uptake and glycolysis in brown adipose tissue.
Albert V, Svensson K, Shimobayashi M, Colombi M, Muñoz S, Jimenez V, Handschin C, Bosch F, Hall MN
EMBO molecular medicine 2016 Mar 1;8(3):232-46
EMBO molecular medicine 2016 Mar 1;8(3):232-46
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Glut4 Antibody (3G10A3) [NBP2-22214] - Western blot analysis using Glucose Transporter GLUT4 mouse mAb against HeLa (1), NIH3T3 (2), 3T3-L1 (3) cell lysate and Mouse heart (4) tissue lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Glut4 Antibody (3G10A3) [NBP2-22214] - Western blot analysis using Glucose Transporter Glut4 Antibody (3G10A3)mAb against HEK293 (1) and Glucose Transporter GLUT4 (AA: 224-353)-hIgGFc transfected HEK293 (2) cell lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Glut4 Antibody (3G10A3) [NBP2-22214] - human Glucose Transporter GLUT4 recombinant protein.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: Glut4 Antibody (3G10A3) [NBP2-22214] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Glut4 Antibody (3G10A3) [NBP2-22214] - Analysis of paraffin-embedded bladder cancer tissues using Glucose Transporter GLUT4 mouse mAb with DAB staining.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Glut4 Antibody (3G10A3) [NBP2-22214] - analysis of cardiac muscle tissues using SLC2A4 mouse mAb with DAB staining.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Glut4 Antibody (3G10A3) [NBP2-22214] - Flow cytometric analysis of HeLa cells using Glucose Transporter GLUT4 mouse mAb (green) and negative control (purple).