MA5-17176

antibody from Invitrogen Antibodies

Targeting: SLC2A4 GLUT4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-17176

Provider

Invitrogen Antibodies

Product name

GLUT4 Monoclonal Antibody (3G10A3)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-17176 targets SLC2A4 in FACS, ICC, IHC, IF and WB applications and shows reactivity with Human and Mouse samples.

Antibody clone number

3G10A3

Concentration

1 mg/mL

References

Submitted references

D-Chiro-Inositol Regulates Insulin Signaling in Human Adipocytes.

Montt-Guevara MM, Finiguerra M, Marzi I, Fidecicchi T, Ferrari A, Genazzani AD, Simoncini T
Frontiers in endocrinology 2021;12:660815

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Chemical systems biology reveals mechanisms of glucocorticoid receptor signaling.

Bruno NE, Nwachukwu JC, Srinivasan S, Nettles CC, Izard T, Jin Z, Nowak J, Cameron MD, Boregowda SV, Phinney DG, Elemento O, Liu X, Ortlund EA, Houtman R, Stavreva DA, Hager GL, Kamenecka TM, Kojetin DJ, Nettles KW
Nature chemical biology 2021 Mar;17(3):307-316

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Validation of an adipose-liver human-on-a-chip model of NAFLD for preclinical therapeutic efficacy evaluation.

Slaughter VL, Rumsey JW, Boone R, Malik D, Cai Y, Sriram NN, Long CJ, McAleer CW, Lambert S, Shuler ML, Hickman JJ
Scientific reports 2021 Jun 23;11(1):13159

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Increased glucose metabolism in Arid5b(-/-) skeletal muscle is associated with the down-regulation of TBC1 domain family member 1 (TBC1D1).

Okazaki Y, Murray J, Ehsani A, Clark J, Whitson RH, Hirose L, Yanaka N, Itakura K
Biological research 2020 Oct 6;53(1):45

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Activating transcription factor 3 attenuates chemokine and cytokine expression in mouse skeletal muscle after exercise and facilitates molecular adaptation to endurance training.

Fernández-Verdejo R, Vanwynsberghe AM, Essaghir A, Demoulin JB, Hai T, Deldicque L, Francaux M
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2017 Feb;31(2):840-851

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SLC2A4 using a SLC2A4 monoclonal antibody (Product # MA5-17176) against a human SLC2A4 recombinant protein.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SLC2A4 using SLC2A4 monoclonal antibody (Product # MA5-17176) in HEK293 (1) and SLC2A4 (AA: 224-353) human IgG Fc transfected HEK293 (2) cell lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SLC2A4 using a SLC2A4 monoclonal antibody (Product # MA5-17176) against a human SLC2A4 recombinant protein.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SLC2A4 using SLC2A4 monoclonal antibody (Product # MA5-17176) in HEK293 (1) and SLC2A4 (AA: 224-353) human IgG Fc transfected HEK293 (2) cell lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SLC2A4 using SLC2A4 monoclonal antibody (Product # MA5-17176) in HeLa (1), NIH3T3 (2), 3T3-L1 (3) cell lysate and mouse heart (4) tissue lysate.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of HeLa cells using SLC2A4 monoclonal antibody (Product # MA5-17176) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of HepG2 cells using SLC2A4 monoclonal antibody (Product # MA5-17176) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using SLC2A4 monoclonal antibody (Product # MA5-17176) followed with DAB staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded cardiac muscle tissues using SLC2A4 monoclonal antibody (Product # MA5-17176) followed with DAB staining.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of HeLa cells using SLC2A4 monoclonal antibody (Product # MA5-17176) (green) and negative control (purple).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 7 D-Chiro-Ins does not modulate the expression of GLUT4. Mature SGBS adipocytes cells were treated with D-Chiro-Ins 10 -8 M, E2 10 -9 M or INS 10 -7 M, and combinations for 24 h. Cell lysates were immunoblotted with an anti-GLUT4 antibody, anti-p-GLUT4 antibody, and anti-GAPDH antibody. (A) Panels show representative blot of GLUT4, p-GLUT4 S488 and GAPDH. (B-D) Analysis of the relative expression level of GLUT4, p-GLUT4 and the ratio p-GLUT4/GLUT4; expression that was normalized with GAPDH and relative to the control. Values are presented as mean +- SEM of three independent experiments. Results were analyzed statistically by one-way RM ANOVA followed by Dunnett''s multiple comparisons test (*p < 0.05, **p < 0.001 versus control). D-Chiro-Ins, D-chiro-Inositol; E2, 17beta-estradiol, INS, insulin; GLUT4, Glucose Transporter 4; p-GLUT4, phospho-GLUT4; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.