Antibody data
- Antibody Data
- Antigen structure
- References [10]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- PB9109 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Glucose Transporter GLUT4/SLC2A4 Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for Glut4/SLC2A4 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. Glut4/SLC2A4 information: Molecular Weight: 54787 MW; Subcellular Localization: Cell membrane ; Multi-pass membrane protein . Endomembrane system ; Multi-pass membrane protein . Cytoplasm, perinuclear region . Localizes primarily to the perinuclear region, undergoing continued recycling to the plasma membrane where it is rapidly reinternalized. The dileucine internalization motif is critical for intracellular sequestration; Tissue Specificity: Skeletal and cardiac muscles; brown and white fat.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Alpha-lipoic Acid Prevents Bone Loss in Type 2 Diabetes and Postmenopausal Osteoporosis Coexisting Conditions by Modulating the YAP/Glut4 Pathway.
Alfuzosin ameliorates diabetes by boosting PGK1 activity in diabetic mice.
Aerobic exercise ameliorates insulin resistance in C57BL/6 J mice via activating Sestrin3.
Ameliorated effects of a lipopeptide surfactin on insulin resistance in vitro and in vivo.
The effects of Formoterol in preventing adipogenesis and obesity are mediated by PPARγ/C/EBPα axis and AMPK/PGC-1α pathway.
Coumarins ameliorate diabetogenic action of dexamethasone via Akt activation and AMPK signaling in skeletal muscle.
Role of Phosphorylated AMP-Activated Protein Kinase (AMPK) in Myocardial Insulin Resistance After Myocardial Ischemia-Reperfusion During Cardiopulmonary Bypass Surgery in Dogs.
Effect of maternal feed restriction in dairy goats at different stages of gestation on skeletal muscle development and energy metabolism of kids at the time of births.
Asiatic acid protests against myocardial ischemia/reperfusion injury via modulation of glycometabolism in rat cardiomyocyte.
Vibration exercise decreases insulin resistance and modulates the insulin signaling pathway in a type 2 diabetic rat model.
Xu L, Zhang C, Bao J, Han G, Wang C, Cai Y, Xu G, Sun H, Liu M
Cell biochemistry and biophysics 2024 Jun;82(2):669-685
Cell biochemistry and biophysics 2024 Jun;82(2):669-685
Alfuzosin ameliorates diabetes by boosting PGK1 activity in diabetic mice.
Zhou J, Wu T, Li C, Hu Z, Han L, Li X, Liu J, Zhao W, Kang J, Chen X
Life sciences 2023 Mar 15;317:121491
Life sciences 2023 Mar 15;317:121491
Aerobic exercise ameliorates insulin resistance in C57BL/6 J mice via activating Sestrin3.
Han X, Yang Y, Liu S, Niu Y, Shao H, Fu L
Biochimica et biophysica acta. Molecular basis of disease 2023 Jan 1;1869(1):166568
Biochimica et biophysica acta. Molecular basis of disease 2023 Jan 1;1869(1):166568
Ameliorated effects of a lipopeptide surfactin on insulin resistance in vitro and in vivo.
Chen X, Zhao H, Meng F, Zhou L, Pang X, Lu Z, Lu Y
Food science & nutrition 2022 Jul;10(7):2455-2469
Food science & nutrition 2022 Jul;10(7):2455-2469
The effects of Formoterol in preventing adipogenesis and obesity are mediated by PPARγ/C/EBPα axis and AMPK/PGC-1α pathway.
Zhang X, Che L, Shan J, Wang Y, Jia Y, Wu H
Bioscience, biotechnology, and biochemistry 2022 Jul 4;
Bioscience, biotechnology, and biochemistry 2022 Jul 4;
Coumarins ameliorate diabetogenic action of dexamethasone via Akt activation and AMPK signaling in skeletal muscle.
Mo Z, Li L, Yu H, Wu Y, Li H
Journal of pharmacological sciences 2019 Mar;139(3):151-157
Journal of pharmacological sciences 2019 Mar;139(3):151-157
Role of Phosphorylated AMP-Activated Protein Kinase (AMPK) in Myocardial Insulin Resistance After Myocardial Ischemia-Reperfusion During Cardiopulmonary Bypass Surgery in Dogs.
Zhang DS, Liang GY, Liu DX, Yu J, Wang F
Medical science monitor : international medical journal of experimental and clinical research 2019 Jun 4;25:4149-4158
Medical science monitor : international medical journal of experimental and clinical research 2019 Jun 4;25:4149-4158
Effect of maternal feed restriction in dairy goats at different stages of gestation on skeletal muscle development and energy metabolism of kids at the time of births.
Costa TC, Moura FH, Souza RO, Lopes MM, Fontes MMS, Serão NVL, Sanglard LP, Du M, Gionbelli MP, Duarte MS
Animal reproduction science 2019 Jul;206:46-59
Animal reproduction science 2019 Jul;206:46-59
Asiatic acid protests against myocardial ischemia/reperfusion injury via modulation of glycometabolism in rat cardiomyocyte.
Dai Y, Wang Z, Quan M, Lv Y, Li Y, Xin HB, Qian Y
Drug design, development and therapy 2018;12:3573-3582
Drug design, development and therapy 2018;12:3573-3582
Vibration exercise decreases insulin resistance and modulates the insulin signaling pathway in a type 2 diabetic rat model.
Liu Y, Liu C, Lu ML, Tang FT, Hou XW, Yang J, Liu T
International journal of clinical and experimental medicine 2015;8(8):13136-44
International journal of clinical and experimental medicine 2015;8(8):13136-44
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of GLUT4 using anti-GLUT4 antibody (PB9109). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: rat cardiac muscle tissue lysate, lane 2: rat skeletal muscle tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLUT4 antigen affinity purified polyclonal antibody (Catalog # PB9109) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GLUT4 at approximately 55KD. The expected band size for GLUT4 is at 55KD.
- Additional image