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- Antibody Data
- Antigen structure
- References [14]
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- Immunocytochemistry [1]
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- Product number
- MAB1262 - Provider product page
- Provider
- R&D Systems
- Product name
- Rat Glut4 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Recognizes an epitope in the cytoplasmic portion of rat Glut4 and has been shown to bind only to the muscle-adipose isoform of the glucose transporter, which is antigenically unique from the other transport proteins (1-3). Recognizes Glut4 in human, monkey, rat, mouse, and rabbit systems. It does not recognize this protein in canine systems. Its ability to bind to glucose transporter from other species has not been tested.
- Reactivity
- Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 1F8
- Vial size
- 200 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C, as supplied. 1 month, 2 to 8 °C under sterile conditions after opening. 6 months, -20 to -70 °C under sterile conditions after opening.
Submitted references The Parkinson's disease-linked Leucine-rich repeat kinase 2 (LRRK2) is required for insulin-stimulated translocation of GLUT4.
3D organoid-derived human glomeruli for personalised podocyte disease modelling and drug screening.
Changes in photoperiod alter Glut4 expression in skeletal muscle of C57BL/6J mice.
HDL and Glut1 inhibition reverse a hypermetabolic state in mouse models of myeloproliferative disorders.
E4orf1: a novel ligand that improves glucose disposal in cell culture.
Nuclear factor-kappaB decoy oligodeoxynucleotides ameliorate impaired glucose tolerance and insulin resistance in mice with cecal ligation and puncture-induced sepsis.
DOC2B: a novel syntaxin-4 binding protein mediating insulin-regulated GLUT4 vesicle fusion in adipocytes.
A transgenic mouse model to study glucose transporter 4myc regulation in skeletal muscle.
Deficiency and inhibition of cathepsin K reduce body weight gain and increase glucose metabolism in mice.
The human glomerular podocyte is a novel target for insulin action.
The human glomerular podocyte is a novel target for insulin action.
Effects of hormone therapy on insulin signaling proteins in skeletal muscle of cynomolgus monkeys.
Ischemic preconditioning activates AMPK in a PKC-dependent manner and induces GLUT4 up-regulation in the late phase of cardioprotection.
Requirement for 3-phosphoinositide-kependent dinase-1 (PDK-1) in insulin-induced glucose uptake in immortalized brown adipocytes.
Funk N, Munz M, Ott T, Brockmann K, Wenninger-Weinzierl A, Kühn R, Vogt-Weisenhorn D, Giesert F, Wurst W, Gasser T, Biskup S
Scientific reports 2019 Mar 14;9(1):4515
Scientific reports 2019 Mar 14;9(1):4515
3D organoid-derived human glomeruli for personalised podocyte disease modelling and drug screening.
Hale LJ, Howden SE, Phipson B, Lonsdale A, Er PX, Ghobrial I, Hosawi S, Wilson S, Lawlor KT, Khan S, Oshlack A, Quinlan C, Lennon R, Little MH
Nature communications 2018 Dec 4;9(1):5167
Nature communications 2018 Dec 4;9(1):5167
Changes in photoperiod alter Glut4 expression in skeletal muscle of C57BL/6J mice.
Tashiro A, Shibata S, Takai Y, Uchiwa T, Furuse M, Yasuo S
Biochemical and biophysical research communications 2017 Mar 25;485(1):82-88
Biochemical and biophysical research communications 2017 Mar 25;485(1):82-88
HDL and Glut1 inhibition reverse a hypermetabolic state in mouse models of myeloproliferative disorders.
Gautier EL, Westerterp M, Bhagwat N, Cremers S, Shih A, Abdel-Wahab O, Lütjohann D, Randolph GJ, Levine RL, Tall AR, Yvan-Charvet L
The Journal of experimental medicine 2013 Feb 11;210(2):339-53
The Journal of experimental medicine 2013 Feb 11;210(2):339-53
E4orf1: a novel ligand that improves glucose disposal in cell culture.
Dhurandhar EJ, Dubuisson O, Mashtalir N, Krishnapuram R, Hegde V, Dhurandhar NV
PloS one 2011;6(8):e23394
PloS one 2011;6(8):e23394
Nuclear factor-kappaB decoy oligodeoxynucleotides ameliorate impaired glucose tolerance and insulin resistance in mice with cecal ligation and puncture-induced sepsis.
Matsuda N, Yamamoto S, Yokoo H, Tobe K, Hattori Y
Critical care medicine 2009 Oct;37(10):2791-9
Critical care medicine 2009 Oct;37(10):2791-9
DOC2B: a novel syntaxin-4 binding protein mediating insulin-regulated GLUT4 vesicle fusion in adipocytes.
Fukuda N, Emoto M, Nakamori Y, Taguchi A, Miyamoto S, Uraki S, Oka Y, Tanizawa Y
Diabetes 2009 Feb;58(2):377-84
Diabetes 2009 Feb;58(2):377-84
A transgenic mouse model to study glucose transporter 4myc regulation in skeletal muscle.
Schertzer JD, Antonescu CN, Bilan PJ, Jain S, Huang X, Liu Z, Bonen A, Klip A
Endocrinology 2009 Apr;150(4):1935-40
Endocrinology 2009 Apr;150(4):1935-40
Deficiency and inhibition of cathepsin K reduce body weight gain and increase glucose metabolism in mice.
Yang M, Sun J, Zhang T, Liu J, Zhang J, Shi MA, Darakhshan F, Guerre-Millo M, Clement K, Gelb BD, Dolgnov G, Shi GP
Arteriosclerosis, thrombosis, and vascular biology 2008 Dec;28(12):2202-8
Arteriosclerosis, thrombosis, and vascular biology 2008 Dec;28(12):2202-8
The human glomerular podocyte is a novel target for insulin action.
Coward RJ, Welsh GI, Yang J, Tasman C, Lennon R, Koziell A, Satchell S, Holman GD, Kerjaschki D, Tavaré JM, Mathieson PW, Saleem MA
Diabetes 2005 Nov;54(11):3095-102
Diabetes 2005 Nov;54(11):3095-102
The human glomerular podocyte is a novel target for insulin action.
Coward RJ, Welsh GI, Yang J, Tasman C, Lennon R, Koziell A, Satchell S, Holman GD, Kerjaschki D, Tavaré JM, Mathieson PW, Saleem MA
Diabetes 2005 Nov;54(11):3095-102
Diabetes 2005 Nov;54(11):3095-102
Effects of hormone therapy on insulin signaling proteins in skeletal muscle of cynomolgus monkeys.
Shadoan MK, Zhang L, Wagner JD
Steroids 2004 May;69(5):313-8
Steroids 2004 May;69(5):313-8
Ischemic preconditioning activates AMPK in a PKC-dependent manner and induces GLUT4 up-regulation in the late phase of cardioprotection.
Nishino Y, Miura T, Miki T, Sakamoto J, Nakamura Y, Ikeda Y, Kobayashi H, Shimamoto K
Cardiovascular research 2004 Feb 15;61(3):610-9
Cardiovascular research 2004 Feb 15;61(3):610-9
Requirement for 3-phosphoinositide-kependent dinase-1 (PDK-1) in insulin-induced glucose uptake in immortalized brown adipocytes.
Sakaue H, Nishizawa A, Ogawa W, Teshigawara K, Mori T, Takashima Y, Noda T, Kasuga M
The Journal of biological chemistry 2003 Oct 3;278(40):38870-4
The Journal of biological chemistry 2003 Oct 3;278(40):38870-4
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Glut4 in L6 Rat Cell Line. Glut4 was detected in immersion fixed L6 rat myoblast cell line (differentiated to muscle) using 10 µg/mL Rat Glut4 Monoclonal Antibody (Catalog # MAB1262) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
