NBP1-49533

Muñoz VR, Gaspar RC, Esteca MV, Baptista IL, Vieira RFL, da Silva ASR, de Moura LP, Cintra DE, Ropelle ER, Pauli JR
Mechanisms of ageing and development 2020 Mar;186:111213

Decreased Metabolic Flexibility in Skeletal Muscle of Rat Fed with a High-Fat Diet Is Recovered by Individual CLA Isomer Supplementation via Converging Protective Mechanisms.

Trinchese G, Cavaliere G, Cimmino F, Catapano A, Carta G, Pirozzi C, Murru E, Lama A, Meli R, Bergamo P, Banni S, Mollica MP
Cells 2020 Mar 29;9(4)

Regulation of Adipogenesis and Lipid Deposits by Collapsin Response Mediator Protein 2.

Chang YH, Tsai JN, Chang SW, Hsu WT, Yang CP, Hsiao CW, Shiau MY
International journal of molecular sciences 2020 Mar 21;21(6)

Effect of Litsea lancifolia Leaf Extract on Glucose Transporter 4 Translocation and Glucose Uptake in 3T3L1 Cell Line.

Alsawalha M, Al-Subaie AM, Al-Jindan RY, Bolla SR, Balakrishna JP, Ravi PK, Gollapalli SSR, Veeraraghavan VP, Pillai AA, Joseph JP, Mohan SK
Journal of pharmacy & bioallied sciences 2019 Jul-Sep;11(3):240-247

Rho-kinase activity is upregulated in the skeletal muscle of aged exercised rats.

Muñoz VR, Gaspar RC, Minuzzi LG, Dos Santos Canciglieri R, da Silva ASR, de Moura LP, Cintra DE, Ropelle ER, Pauli JR
Experimental gerontology 2019 Dec;128:110746

Exercise increases Rho-kinase activity and insulin signaling in skeletal muscle.

Muñoz VR, Gaspar RC, Kuga GK, da Rocha AL, Crisol BM, Botezelli JD, Baptista IL, Mekary RA, da Silva ASR, Cintra DE, de Moura LP, Ropelle ER, Pauli JR
Journal of cellular physiology 2018 Jun;233(6):4791-4800

Germinated Waxy Black Rice Ameliorates Hyperglycemia and Dyslipidemia in Streptozotocin-Induced Diabetic Rats.

Kang HW, Lim WC, Lee JK, Ho JN, Lim EJ, Cho HY
Biological & pharmaceutical bulletin 2017;40(11):1846-1855

Insulin signaling via the PI3-kinase/Akt pathway regulates airway glucose uptake and barrier function in a CFTR-dependent manner.

Molina SA, Moriarty HK, Infield DT, Imhoff BR, Vance RJ, Kim AH, Hansen JM, Hunt WR, Koval M, McCarty NA
American journal of physiology. Lung cellular and molecular physiology 2017 May 1;312(5):L688-L702

Innovative approach to safely induce controlled lipolysis by superparamagnetic iron oxide nanoparticles-mediated hyperthermic treatment.

Marinozzi MR, Pandolfi L, Malatesta M, Colombo M, Collico V, Lievens PM, Tambalo S, Lasconi C, Vurro F, Boschi F, Mannucci S, Sbarbati A, Prosperi D, Calderan L
The international journal of biochemistry & cell biology 2017 Dec;93:62-73

Co-activator binding protein PIMT mediates TNF-α induced insulin resistance in skeletal muscle via the transcriptional down-regulation of MEF2A and GLUT4.

Kain V, Kapadia B, Viswakarma N, Seshadri S, Prajapati B, Jena PK, Teja Meda CL, Subramanian M, Kaimal Suraj S, Kumar ST, Prakash Babu P, Thimmapaya B, Reddy JK, Parsa KV, Misra P
Scientific reports 2015 Oct 15;5:15197

Glucagon-like peptide 1 (GLP-1)-based therapy upregulates LXR-ABCA1/ABCG1 cascade in adipocytes.

Mostafa AM, Hamdy NM, El-Mesallamy HO, Abdel-Rahman SZ
Biochemical and biophysical research communications 2015 Dec 25;468(4):900-5

Deficiency in mTORC1-controlled C/EBPβ-mRNA translation improves metabolic health in mice.

Zidek LM, Ackermann T, Hartleben G, Eichwald S, Kortman G, Kiehntopf M, Leutz A, Sonenberg N, Wang ZQ, von Maltzahn J, Müller C, Calkhoven CF
EMBO reports 2015 Aug;16(8):1022-36

Mice deficient in Sfrp1 exhibit increased adiposity, dysregulated glucose metabolism, and enhanced macrophage infiltration.

Gauger KJ, Bassa LM, Henchey EM, Wyman J, Bentley B, Brown M, Shimono A, Schneider SS
PloS one 2013;8(12):e78320

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Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Glut4 Antibody [NBP1-49533] - Analysis of GLUT4 in A) MCF7 whole cell lysate and B) 3T3L1 whole cell lysate.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Glut4 Antibody [NBP1-49533] - Total protein from Human HeLa and A431, Mouse 3T3 and Rat PC12 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-Glut4 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Glut4 Antibody [NBP1-49533] - Total protein from 3T3-L1 mouse embryonic fibroblast adipose-like cell line, separated by 4-12% SDS-PAGE, transferred to nitrocellulose membrane and blocked in 5% non-fat milk for 1h at room temperature. The membrane was probed with anti-Glut4 0.5 ug/ml in non-fat milk. Undiffer: undifferentiated; Differ: Differentiated. Image from verified customer review.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Immunohistochemistry: Glut4 Antibody [NBP1-49533] - Analysis of GLUT4 in mouse kidney

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: Glut4 Antibody [NBP1-49533] - GLUT4 Biotin/APC vs. [X] PE. Image from verified customer review.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow Cytometry: Glut4 Antibody [NBP1-49533] - Analysis using the Alexa Fluor (R) 647 conjugate of NBP1-49533. Staining of Glut 4 expression on Murine CD4+ T cells stimulated with anti-CD3/CD28 beads and insulin (1ug/mL) for 5 days in culture media with additional glucose provided. This Alexa Fluor (R) 647 conjugated Glut 4 antibody (orange) positively stained mouse CD4+ T cells compared to Isotype Control (Rb IgG AF647, Novus NBP2-36463AF647, blue) and fluorescence minus one/FMO control (red). Image from verified customer review.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Flow (Intracellular): Glut4 Antibody [NBP1-49533] - An intracellular stain was performed on HepG2 with NBP1-49533 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody.