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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-29393 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CDK5RAP1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: U87-MG, SK-N-SH, IMR32, SK-N-AS.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CDK5RAP1 using 30 µg of HeLa lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a CDK5RAP1 polyclonal antibody (Product # PA5-29393) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CDK5RAP1 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a CDK5RAP1 Polyclonal Antibody (Product # PA5-29393) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of CDK5RAP1 in paraformaldehyde-fixed HeLa cells using a CDK5RAP1 polyclonal antibody (Product # PA5-29393) at a 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of CDK5RAP1 was performed in paraffin-embedded Rat brain tissue using CDK5RAP1 Polyclonal Antibody (Product # PA5-29393) at a dilution of 1:500.
