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LearnNBP2-21648
antibody from Novus Biologicals
Targeting: AP2A2
ADTAB, CLAPA2, DKFZP564D1864, HIP9, HYPJ, KIAA0899
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
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- Product number
- NBP2-21648 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal AP2A2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat, Canine, Chicken/Avian
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.7 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: AP2A2 Antibody [NBP2-21648] - Affinity Purified anti-AP2A antibody shows detection of a band just below 100 kDa corresponding to Human AP2A1 in a various preparations. Lane 1: HeLa nuclear extract, Lane 2: HeLa, Lane 3: 293, Lane 4: A431 and Lane 5: Jurkat whole cell lysates. In lanes 6-10 the antibody was preincubated with 1 ug/ml of the immunizing peptide which effectively blocks the specific reactivity of this antibody with AP2A. Approx 20 ug of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-AP2A antibody. Detection occurred using a 1:5000 dilution of HRP-labeled Rabbit anti-Goat IgG for 1 hour at room temperature. A chemi-luminescence system was used for signal detection using a 60-sec exposure time.
