PA5-117785

antibody from Invitrogen Antibodies

Targeting: AP2A2 ADTAB, CLAPA2, DKFZP564D1864, HIP9, HYPJ, KIAA0899

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-117785

Provider

Invitrogen Antibodies

Product name

AP2A2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using AP2A2 Polyclonal Antibody (Product # PA5-117785) at 1:500 dilution. Lane A: RT-4 Whole Cell Lysate, Lane B: U-251 MG Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 104 kDa. Observed band size: 104 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using AP2A2 Polyclonal Antibody (Product # PA5-117785) at 1:500 dilution. Lane A: RT-4 Whole Cell Lysate, Lane B: U-251 MG Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 104 kDa. Observed band size: 104 kDa.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human AP2A2 in human rectum with AP2A2 Polyclonal Antibody (Product # PA5-117785, 1:100 dilution, formalin-fixed paraffin embedded sections).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human AP2A2 in human testis with AP2A2 Polyclonal Antibody (Product # PA5-117785, 1:100 dilution, formalin-fixed paraffin embedded sections).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

AP2A2 Immunoprecipitation using: Lane A: 0.5 mg RT-4 Whole Cell Lysate 4 µL with AP2A2 Polyclonal Antibody (Product # PA5-117785) and 60 μg of Immunomagnetic beads Protein A/G. Primary antibody: AP2A2 Polyclonal Antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) /HRP at 1:10,000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 104 kDa. Observed band size: 104 kDa.