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LearnAP09207PU-N
antibody from Acris Antibodies GmbH
Targeting: AP2A2
ADTAB, CLAPA2, DKFZP564D1864, HIP9, HYPJ, KIAA0899
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- AP09207PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP09207PU-N, RRID:AB_2035128
- Product name
- anti AP2A2 / HIP9 (3-14)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide corresponding aa 3-14 of Human AP2A1 and AP2A2 proteins
- Reactivity
- Human, Mouse, Rat, Canine, Chicken/Avian
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.7 mg/ml (by UV absorbance at 280 nm)
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot using Affinity Purified anti-AP2A antibody shows detection of a band just below 100 kDa corresponding to Human AP2A1 in a various preparations. Lane 1 - HeLa nuclear extract, Lane 2 - HeLa, Lane 3 - 293, Lane 4 - A431 and Lane 5 - Jurkat whole cell lysates. In lanes 6-10 the antibody was preincubated with 1 µg/ml of the immunizing peptide which effectively blocks the specific reactivity of this antibody with AP2A. Approximately 20 µg of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-AP2A antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Rabbit anti-Goat IgG for 1 hour at room temperature. A chemi-luminescence system was used for signal detection (Roche) using a 60-sec exposure time.
