Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- BS60356 - Provider product page
- Provider
- Bioworld Technology, Inc
- Product name
- CD3G polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- A synthetic peptide corresponding to residues in Human CD3G.
- Description
- The T cell antigen receptor (TCR) recognizesforeign antigens and translates such recognition eventsinto intracellularsignalsthat elicit a change in the cell froma dormant to an activated state.Much of this signaling processcan be attributed to a multisubunitcomplex of proteins that associates directly with the TCR. Thiscomplex has been designated CD3 (cluster of differentiation 3). It iscomposed of five invariant polypeptide chainsthat associate to form three dimers: a heterodimer of ÎÛ and ÷ chains(ÎÛ÷), a heterodimer of Ñ´ and ÷ chains(Ñ´÷) and a homodimer of two ø chains(øø) or a heterodimer of ø and »Ò chains(ø»Ò). The ø and »Ò chains are encoded bythe same gene but differ in theircarboxyl-terminal ends due to an alternative splicing event. The ÎÛ, ÷ and Ñ´ chains each contain a single copy of a conserved immunoreceptor tyrosine-based activationmotif (ITAM). In contrast, the ø chain containsthree consecutive copies of the same motif. Phosphorylated ITAMs act as docking sitesfor protein kinases such as ZAP-70 and Syk and are also capable of regulating theirkinase activity. The crystalstructure of ZAP-70£ªs SH2 domains bound to the ø chain ITAMs has been solved.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Bioworld Technology, Inc (provider)
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- Experimental details
- Western blot (WB) analysis of CD3G pAb at 1:500 dilutionLane1:HEK293T whole cell lysateLane2:Raw264.7 whole cell lysateLane3:H9C2 whole cell lysate