629402

antibody from BioLegend
Targeting: NEK2 NEK2A, NLK1, PPP1R111, RP67
Provider product page for 629402

Antibody data

Product number
629402 - Provider product page
Provider
BioLegend
Proper citation
BioLegend Cat#629402, RRID:AB_2150374
Product name
Purified anti-NEK2
Antibody type
Monoclonal
Antigen
Recombinant protein
Description
NEK2 is a serine/threonine kinase involved in mitotic regulation. NEK2 also known as serine/threonine protein kinase Nek2, NimA-related protein kinase 2, and HSPK21, can be expressed as 3 different isoforms as a result of alternative splicing.. The predicted molecular weight of full-length protein is approximately 52 kD. Isoform 1 and isoform 2 forms homo- and heterodimers; isoform 1 has a nucleolar targeting/retention signal in C-terminal domain. Isoform 2 is missing the C-terminal sequence and remains mainly in the cytoplasm. Isoform 1 plays a role in centrosome splitting and phosphorylates and activates NEK11 is G1/S arrested cells. NEK2 accumulates throughout S phase, becoming maximal in late G2 with expression similar to that of A and B cyclins. Isoform 1 is not present in cells arrested in G2/M prometaphase. Both isoform 1 and 2 are expressed in peripheral T cells and many other transformed cell types. The NEK2 protein has been shown to interact with the TERF1 protein. The 8A6 monoclonal antibody recognizes human NEK2 and is useful for Western blotting.
Reactivity
Human
Host
Mouse
Conjugate
Unconjugated
Isotype
IgG
Vial size
100 µg
Concentration
0.5 mg/ml
Storage
Upon receipt, store undiluted at 4° C.
NEK2 protein structure - 629402 shown in red.