Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-31259 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NEK2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T.
- Concentration
- 1 mg/mL
Submitted references NEK2 inhibition triggers anti-pancreatic cancer immunity by targeting PD-L1.
Targeting NEK2 attenuates glioblastoma growth and radioresistance by destabilizing histone methyltransferase EZH2.
Zhang X, Huang X, Xu J, Li E, Lao M, Tang T, Zhang G, Guo C, Zhang X, Chen W, Yadav DK, Bai X, Liang T
Nature communications 2021 Jul 27;12(1):4536
Nature communications 2021 Jul 27;12(1):4536
Targeting NEK2 attenuates glioblastoma growth and radioresistance by destabilizing histone methyltransferase EZH2.
Wang J, Cheng P, Pavlyukov MS, Yu H, Zhang Z, Kim SH, Minata M, Mohyeldin A, Xie W, Chen D, Goidts V, Frett B, Hu W, Li H, Shin YJ, Lee Y, Nam DH, Kornblum HI, Wang M, Nakano I
The Journal of clinical investigation 2017 Aug 1;127(8):3075-3089
The Journal of clinical investigation 2017 Aug 1;127(8):3075-3089
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using NEK2 Polyclonal Antibody (Product # PA5-31259). Sample (30 µg of whole cell lysate). Lane A: 293T. 10% SDS PAGE. NEK2 Polyclonal Antibody (Product # PA5-31259) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of NEK2 in paraformaldehyde-fixed HeLa cells using a NEK2 polyclonal antibody (Product # PA5-31259) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NEK2 Polyclonal Antibody detects NEK2 protein at cytoplasm and nucleus on human ovarian carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian carcinoma. NEK2 Polyclonal Antibody (Product # PA5-31259) diluted at 1:250. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.