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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- ARG53998 - Provider product page
- Provider
- Arigo
- Product name
- anti-HP1 alpha antibody
- Antibody type
- Monoclonal
- Antigen
- Purified recombinant human HP1 alpha protein fragments expressed in E.coli.
- Description
- Affinity purified
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 3G2-H10-A6
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
- Storage
- Aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: HP1 alpha in MCF7, 293T, HeLaNE, 3T3, C6, COS7 and Mouse brain lysates and stained with HP1 alpha antibody [3G2-H10-A6] (ARG53998) (diluted in 1: 1000). Predicted band size: 22KDa. Observed band size: 22KDa
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunocytochemistry stain of HeLa using HP1 alpha antibody [3G2-H10-A6] (ARG53998) (diluted in 1:300).
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: paraffin-embedded human breast carcinoma with HP1 alpha antibody [3G2-H10-A6] (ARG53998) (1:400 diluted) , showing nuclear localization. A high pressure mediated antigen retrieval step was performed in citrat buffer (pH6. 0).
