H00023468-M01

antibody from Abnova Corporation

Targeting: CBX5 HP1, HP1-ALPHA, HP1Hs-alpha

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00023468-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00023468-M01, RRID:AB_425906

Product name

CBX5 monoclonal antibody (M01), clone 1E11-3A10

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant CBX5.

Antigen sequence

MGKKTKRTADSSSSEDEEEYVVEKVLDRRVVKGQV
EYLLKWKGFSEEHNTWEPEKNLDCPELISEFMKKY
KKMKEGENNKPREKSESNKRKSNFSNSADDIKSKK
KREQSNDIARGFERGLEPEKIIGATDSCGDLMFLM
KWKDTDEADLVLAKEANVKCPQIVIAFYEERLTWH
AYPEDAENKEKETAKS


Isotype

IgG

Antibody clone number

1E11-3A10

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

CBX5 monoclonal antibody (M01), clone 1E11-3A10 Western Blot analysis of CBX5 expression in Hela S3 NE ( Cat # L013V3 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of CBX5 expression in transfected 293T cell line by CBX5 monoclonal antibody (M01), clone 1E11-3A10.Lane 1: CBX5 transfected lysate(22.2 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged CBX5 is approximately 0.03ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to CBX5 on HeLa cell. [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol