Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX107405 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX107405, RRID:AB_11168810
- Product name
- PDK1 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Storage
- Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Submitted references Homoharringtonine induces apoptosis and inhibits STAT3 via IL-6/JAK1/STAT3 signal pathway in Gefitinib-resistant lung cancer cells.
Cao W, Liu Y, Zhang R, Zhang B, Wang T, Zhu X, Mei L, Chen H, Zhang H, Ming P, Huang L
Scientific reports 2015 Jul 13;5:8477
Scientific reports 2015 Jul 13;5:8477
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PDK1 antibody detects PDK1 protein by western blot analysis.A. 30 μg 293T whole cell extract B. 30 μg A431 whole cell extract 10 % SDS-PAGEPDK1 antibody (GTX107405) dilution: 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (50 μg of whole cell lysate) A: mouse heart 10% SDS PAGE GTX107405 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PDK1 antibody detects PDK1 protein by western blot analysis.A. 30 μg 293T whole cell extract B. 30 μg whole cell extract of human PDK1 transfected 293T cells10 % SDS-PAGEPDK1 antibody (GTX107405) dilution: 1:10000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed HeLa, using PDK1(GTX107405) antibody at 1:500 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of PDK1 protein from 293T whole cell extracts using 5 £gg of PDK1 antibody (GTX107405).Western blot analysis was performed using PDK1 antibody (GTX107405).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
