Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NB100-82137 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-82137, RRID:AB_1144596
- Product name
- Rabbit Polyclonal PDK-1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. The antibody detects endogenous level of PDK1 only when phosphorylated at serine 241
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.05 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PDK-1 [p Ser241] Antibody [NB100-82137] - Analysis of extracts from 293 and PC12 cells untreated or treated with EGF using PDK1(Phospho-Ser241) Antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PDK-1 [p Ser241] Antibody [NB100-82137] - Analysis of extracts from MDA-MB-435 cells using PDK1 (phospho-Ser241) antibody NB100-82137.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: PDK-1 [p Ser241] Antibody [NB100-82137] - Staining of methanol-fixed Hela cells.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PDK-1 [p Ser241] Antibody [NB100-82137] - Analysis of paraffin- embedded human breast carcinoma tissue using PDK1 (phospho-Ser241) antibody NB100-82137.