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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- AP21768PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP21768PU-N, RRID:AB_10757538
- Product name
- anti EIF3B / EIF3S9
- Antibody type
- Polyclonal
- Antigen
- A portion of amino acids 1-50 of human eIF3B
- Reactivity
- Human, Mouse, Simian
- Host
- Rabbit
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of eIF3B in A431 cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) NIH 3T3 cell lysate using AP21768PU-N at 0.25 µg/ml, 0.25 µg/ml and 0.05 µg/ml respectively.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of eIF3B in formalin-fixed, paraffin-embedded human thyroid tissue using an isotype control (top left) and AP21768PU-N (bottom left, right) at 5 µg/ml.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded human brain tissue stained with eIF3B antibody (Cat.-No.: AP21768PU-N) at 10 µg/ml.Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
