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antibody from R&D Systems
Targeting: CXCL12
PBSF, SCYB12, SDF-1a, SDF-1b, SDF1, SDF1A, SDF1B, TLSF-a, TLSF-b, TPAR1
Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [17]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF-310-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Human CXCL12/SDF-1 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human CXCL12/SDF-1 in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q6ICW0- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Receptor-Ligand Interaction Mediates Targeting of Endothelial Colony Forming Cell-derived Exosomes to the Kidney after Ischemic Injury.
Dual targeting of the chemokine receptors CXCR4 and ACKR3 with novel engineered chemokines.
A paracrine network regulates the cross-talk between human lung stem cells and the stroma.
Lymphatic specific disruption in the fine structure of heparan sulfate inhibits dendritic cell traffic and functional T cell responses in the lymph node.
COUP-TFI modifies CXCL12 and CXCR4 expression by activating EGF signaling and stimulates breast cancer cell migration.
Identification and characterization of circulating variants of CXCL12 from human plasma: effects on chemotaxis and mobilization of hematopoietic stem and progenitor cells.
CXCL12 is essential for migration of activated Langerhans cells from epidermis to dermis.
Participation of stem cells from human cord blood in skeletal muscle regeneration of SCID mice.
Functional gene expression analysis of clonal plasma cells identifies a unique molecular profile for light chain amyloidosis.
CC chemokine ligands 25 and 28 play essential roles in intestinal extravasation of IgA antibody-secreting cells.
CC chemokine ligands 25 and 28 play essential roles in intestinal extravasation of IgA antibody-secreting cells.
A transmigratory cup in leukocyte diapedesis both through individual vascular endothelial cells and between them.
Recapitulation of B cell differentiation in the central nervous system of patients with multiple sclerosis.
Phenotypic and functional analysis of T cells homing into the CSF of subjects with inflammatory diseases of the CNS.
Phenotypic and functional analysis of T cells homing into the CSF of subjects with inflammatory diseases of the CNS.
Stromal-derived factor 1 inhibits the cycling of very primitive human hematopoietic cells in vitro and in NOD/SCID mice.
Stromal-derived factor 1 inhibits the cycling of very primitive human hematopoietic cells in vitro and in NOD/SCID mice.
Viñas JL, Spence M, Gutsol A, Knoll W, Burger D, Zimpelmann J, Allan DS, Burns KD
Scientific reports 2018 Nov 5;8(1):16320
Scientific reports 2018 Nov 5;8(1):16320
Dual targeting of the chemokine receptors CXCR4 and ACKR3 with novel engineered chemokines.
Hanes MS, Salanga CL, Chowdry AB, Comerford I, McColl SR, Kufareva I, Handel TM
The Journal of biological chemistry 2015 Sep 11;290(37):22385-97
The Journal of biological chemistry 2015 Sep 11;290(37):22385-97
A paracrine network regulates the cross-talk between human lung stem cells and the stroma.
Ruiz EJ, Oeztuerk-Winder F, Ventura JJ
Nature communications 2014;5:3175
Nature communications 2014;5:3175
Lymphatic specific disruption in the fine structure of heparan sulfate inhibits dendritic cell traffic and functional T cell responses in the lymph node.
Yin X, Johns SC, Kim D, Mikulski Z, Salanga CL, Handel TM, Macal M, Zúñiga EI, Fuster MM
Journal of immunology (Baltimore, Md. : 1950) 2014 Mar 1;192(5):2133-42
Journal of immunology (Baltimore, Md. : 1950) 2014 Mar 1;192(5):2133-42
COUP-TFI modifies CXCL12 and CXCR4 expression by activating EGF signaling and stimulates breast cancer cell migration.
Boudot A, Kerdivel G, Lecomte S, Flouriot G, Desille M, Godey F, Leveque J, Tas P, Le Dréan Y, Pakdel F
BMC cancer 2014 Jun 6;14:407
BMC cancer 2014 Jun 6;14:407
Identification and characterization of circulating variants of CXCL12 from human plasma: effects on chemotaxis and mobilization of hematopoietic stem and progenitor cells.
Richter R, Jochheim-Richter A, Ciuculescu F, Kollar K, Seifried E, Forssmann U, Verzijl D, Smit MJ, Blanchet X, von Hundelshausen P, Weber C, Forssmann WG, Henschler R
Stem cells and development 2014 Aug 15;23(16):1959-74
Stem cells and development 2014 Aug 15;23(16):1959-74
CXCL12 is essential for migration of activated Langerhans cells from epidermis to dermis.
Ouwehand K, Santegoets SJ, Bruynzeel DP, Scheper RJ, de Gruijl TD, Gibbs S
European journal of immunology 2008 Nov;38(11):3050-9
European journal of immunology 2008 Nov;38(11):3050-9
Participation of stem cells from human cord blood in skeletal muscle regeneration of SCID mice.
Brzóska E, Grabowska I, Hoser G, Stremińska W, Wasilewska D, Machaj EK, Pojda Z, Moraczewski J, Kawiak J
Experimental hematology 2006 Sep;34(9):1262-70
Experimental hematology 2006 Sep;34(9):1262-70
Functional gene expression analysis of clonal plasma cells identifies a unique molecular profile for light chain amyloidosis.
Abraham RS, Ballman KV, Dispenzieri A, Grill DE, Manske MK, Price-Troska TL, Paz NG, Gertz MA, Fonseca R
Blood 2005 Jan 15;105(2):794-803
Blood 2005 Jan 15;105(2):794-803
CC chemokine ligands 25 and 28 play essential roles in intestinal extravasation of IgA antibody-secreting cells.
Hieshima K, Kawasaki Y, Hanamoto H, Nakayama T, Nagakubo D, Kanamaru A, Yoshie O
Journal of immunology (Baltimore, Md. : 1950) 2004 Sep 15;173(6):3668-75
Journal of immunology (Baltimore, Md. : 1950) 2004 Sep 15;173(6):3668-75
CC chemokine ligands 25 and 28 play essential roles in intestinal extravasation of IgA antibody-secreting cells.
Hieshima K, Kawasaki Y, Hanamoto H, Nakayama T, Nagakubo D, Kanamaru A, Yoshie O
Journal of immunology (Baltimore, Md. : 1950) 2004 Sep 15;173(6):3668-75
Journal of immunology (Baltimore, Md. : 1950) 2004 Sep 15;173(6):3668-75
A transmigratory cup in leukocyte diapedesis both through individual vascular endothelial cells and between them.
Carman CV, Springer TA
The Journal of cell biology 2004 Oct 25;167(2):377-88
The Journal of cell biology 2004 Oct 25;167(2):377-88
Recapitulation of B cell differentiation in the central nervous system of patients with multiple sclerosis.
Corcione A, Casazza S, Ferretti E, Giunti D, Zappia E, Pistorio A, Gambini C, Mancardi GL, Uccelli A, Pistoia V
Proceedings of the National Academy of Sciences of the United States of America 2004 Jul 27;101(30):11064-9
Proceedings of the National Academy of Sciences of the United States of America 2004 Jul 27;101(30):11064-9
Phenotypic and functional analysis of T cells homing into the CSF of subjects with inflammatory diseases of the CNS.
Giunti D, Borsellino G, Benelli R, Marchese M, Capello E, Valle MT, Pedemonte E, Noonan D, Albini A, Bernardi G, Mancardi GL, Battistini L, Uccelli A
Journal of leukocyte biology 2003 May;73(5):584-90
Journal of leukocyte biology 2003 May;73(5):584-90
Phenotypic and functional analysis of T cells homing into the CSF of subjects with inflammatory diseases of the CNS.
Giunti D, Borsellino G, Benelli R, Marchese M, Capello E, Valle MT, Pedemonte E, Noonan D, Albini A, Bernardi G, Mancardi GL, Battistini L, Uccelli A
Journal of leukocyte biology 2003 May;73(5):584-90
Journal of leukocyte biology 2003 May;73(5):584-90
Stromal-derived factor 1 inhibits the cycling of very primitive human hematopoietic cells in vitro and in NOD/SCID mice.
Cashman J, Clark-Lewis I, Eaves A, Eaves C
Blood 2002 Feb 1;99(3):792-9
Blood 2002 Feb 1;99(3):792-9
Stromal-derived factor 1 inhibits the cycling of very primitive human hematopoietic cells in vitro and in NOD/SCID mice.
Cashman J, Clark-Lewis I, Eaves A, Eaves C
Blood 2002 Feb 1;99(3):792-9
Blood 2002 Feb 1;99(3):792-9
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Chemotaxis Induced by CXCL12/SDF-1 alpha and Neutralization by Human CXCL12/SDF-1 Antibody. Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF-1 alpha (Catalog # 350-NS) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF-1 alpha (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CXCL12/SDF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-310-NA). The ND50 is typically 1.5-6.0 µg/mL.
