PA5-78599

antibody from Invitrogen Antibodies

Targeting: MED10 L6, MGC5309, NUT2, TRG20

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-78599

Provider

Invitrogen Antibodies

Product name

MED10 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive Control: 293T, 293T nuclear extract

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of MED10 in 293T cells using 30 µg of protein. Samples were separated with 15% SDS-PAGE and incubated with MED10 polyclonal antibody (Product # PA5-78599) using a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using MED10 Polyclonal Antibody (Product # PA5-78599). 293T whole cell and nuclear extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with MED10 Polyclonal Antibody (Product # PA5-78599) diluted at 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using MED10 Polyclonal Antibody (Product # PA5-78599). 293T whole cell and nuclear extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with MED10 Polyclonal Antibody (Product # PA5-78599) diluted at 1:500.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of MED10 in 293T cells. Samples were treated with 4% paraformaldehyde at RT for 15 min and incubated with MED10 polyclonal antibody (Product # PA5-78599) using a dilution of 1:500, followed by Hoechst.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MED10 Polyclonal Antibody detects MED10 protein at nucleus by immunofluorescent analysis. Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MED10 protein stained by MED10 Polyclonal Antibody (Product # PA5-78599) diluted at 1:500. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MED10 Polyclonal Antibody detects MED10 protein at nucleus by immunofluorescent analysis. Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MED10 protein stained by MED10 Polyclonal Antibody (Product # PA5-78599) diluted at 1:500. Blue: Hoechst 33342 staining.