Explore
Validate
Learn
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 100-401-402 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#100-401-402, RRID:AB_2273933
- Product name
- Anti-Angiopoietin 2 (RABBIT) Antibody - 100-401-402
- Antibody type
- Polyclonal
- Vial size
- 200 µl
No comments: Submit comment
Supportive validation
- Submitted by
- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Immunocytochemistry of anti-ANG2 Antibody in PC12 cells Tissue: Human prostate cancer cell line (PC12) Fixation: 5 min in 100% methanol Ag Retrieval/permeabilization: 1hr 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween Primary antibody: 1:200 dilution overnight at +4°C Secondary Ab: 488 Gt anti rabbit 1:1000 1 hr RT Staining: Ang2 stained yellow/green, plasma membranes stained red, cell nuclei stained blue with DAPI (1.43 µM)
- Sample type
- Immunocytochemistry
