MAB098-100

antibody from R&D Systems

Targeting: ANGPT2 Ang2

ELISA (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB098-100

Provider

R&D Systems

Product name

Human/Mouse Angiopoietin-2 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human and mouse Angiopoietin-2 in ELISAs. In ELISAs, no cross-reactivity or interference with recombinant human (rh) Ang-1, rhAng-4, rhAng-X, recombinant mouse (rm) Ang-3, rmANGPTL-3, rhTie-1, rhTie-2, and rmTie-2 is observed.

Reactivity

Human, Mouse

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

O15123


Isotype

IgG

Antibody clone number

85816

Vial size

100 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Predictive Value of Clinical Findings and Plasma Biomarkers after Fourteen Days of Prednisone Treatment for Acute Graft-versus-host Disease.

McDonald GB, Tabellini L, Storer BE, Martin PJ, Lawler RL, Rosinski SL, Schoch HG, Hansen JA
Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation 2017 Aug;23(8):1257-1263

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Endothelial exocytosis of angiopoietin-2 resulting from CCM3 deficiency contributes to cerebral cavernous malformation.

Jenny Zhou H, Qin L, Zhang H, Tang W, Ji W, He Y, Liang X, Wang Z, Yuan Q, Vortmeyer A, Toomre D, Fuh G, Yan M, Kluger MS, Wu D, Min W
Nature medicine 2016 Sep;22(9):1033-1042

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Kaposi's sarcoma-associated herpesvirus-encoded interleukin-6 and G-protein-coupled receptor regulate angiopoietin-2 expression in lymphatic endothelial cells.

Vart RJ, Nikitenko LL, Lagos D, Trotter MW, Cannon M, Bourboulia D, Gratrix F, Takeuchi Y, Boshoff C
Cancer research 2007 May 1;67(9):4042-51

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Hyperoxia causes angiopoietin 2-mediated acute lung injury and necrotic cell death.

Bhandari V, Choo-Wing R, Lee CG, Zhu Z, Nedrelow JH, Chupp GL, Zhang X, Matthay MA, Ware LB, Homer RJ, Lee PJ, Geick A, de Fougerolles AR, Elias JA
Nature medicine 2006 Nov;12(11):1286-93

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Angiopoietin-2 in Human Hepatocellular Carcinoma Tissue. Angiopoietin-2 was detected in immersion fixed paraffin-embedded sections of human hepatocellular carcinoma tissue (right panel; positive stain) using Mouse Anti-Human Angiopoietin-2 Monoclonal Antibody (Catalog # MAB098) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to secreted and plasma membrane. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.