Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- LS-B45 - Provider product page
- Provider
- LSBio
- Proper citation
- LifeSpan Cat#LS-B45, RRID:AB_2165762
- Product name
- IHC-plus™ PPARD / PPAR Delta Antibody LS-B45
- Antibody type
- Polyclonal
- Description
- Affinity purified
- Reactivity
- Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Submitted references Transcript profiling identifies dynamic gene expression patterns and an important role for Nrf2/Keap1 pathway in the developing mouse esophagus.
Chen H, Li J, Li H, Hu Y, Tevebaugh W, Yamamoto M, Que J, Chen X
PloS one 2012;7(5):e36504
PloS one 2012;7(5):e36504
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Anti-PPAR Delta Antibody - Western Blot. Affinity Purified Anti-PPAR delta [Rabbit] is shown to detect a predominant band at ~43 kD corresponding to PPAR delta present in mouse heart whole cell lysates. Preincubation of the antibody with the immunizing peptide completely blocks reactivity with this band (data not shown). Approximately 30 ug of lysate was loaded per lane for SDS-PAGE. Detection occurred using a 1:750 dilution of primary antibody diluted in 5% BLOTTO in PBS overnight at 4C followed reaction with a 1:10000 dilution of IRDye 800 conjugated Gt-a-Rabbit IgG (H&L) ( for 45 min at room temperature (800 nm channel, green). IRDye 800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.